RSC - Anal. Methods latest articles

Simultaneous electrochemical detection of dopamine and paracetamol using a modified electrode with hydrothermal treatment of neodymium oxide and carbon paste

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY02082D, Paper

gloria cosco, Eduardo Salazar, Johisner Penagos-Llanos, Rodrigo Segura, Edgar Nagles, John Hurtado Belalcazar
In this report, the modification of the carbon-based electrode with neodymium oxide after hydrothermal treatment led to a significant increase in the electroactive and conductive properties of the electrode. The...
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Selective interaction of As(III) and luminol for the development of electrochemiluminescence-based arsenic(III) sensors

Anal. Methods, 2026, 18,1795-1799
DOI: 10.1039/D5AY02142A, Communication

Harmesa Harmesa, A'an J. Wahyudi, Asep Saefumillah, Andrea Fiorani, Yasuaki Einaga, Tribidasari A. Ivandini
A newly identified selective interaction between As(III) and luminol enhances the formation of excited 3-aminophthalate, thereby amplifying the ECL signals.
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Sensing element design in optical pesticide-detecting arrays

Anal. Methods, 2026, 18,1767-1794
DOI: 10.1039/D5AY01971K, Critical Review

M. Reza Nassajan, Forough Ghasemi
This review elucidates optical sensor array design principles for pesticide detection by analyzing six core classes of sensing elements: label-free nanoparticles, chemosensors, host–guest systems, enzymes, antibodies, and aptamers.
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A one-step chemical derivatization strategy for mass spectrometric characterization of synthetic mimetics of sulfated glycosaminoglycans

Anal. Methods, 2026, 18,1800-1803
DOI: 10.1039/D5AY01855B, Communication

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Chiranjeev Sharma, Daniel K. Afosah, Bharath K. Villuri, Umesh R. Desai
We present a novel one-step methodology for direct mass spectrometric characterization of sulfated non-saccharide glycosaminoglycan mimetics in their sodiated form using trifluorodiazoethane that enables separation and detection in a LC-MS system.
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SERS-based immunochromatographic assay with AuDTNB@Ag nanoparticles for the detection of ancient silk residues

Anal. Methods, 2026, 18,1948-1958
DOI: 10.1039/D5AY02098K, Paper

Xinyu Wen, Ruixue Sun, Hui Si, Junting Li, Chao Zhang, Hailing Zheng, Yang Zhou, Bing Wang
In this research, a surface-enhanced Raman scattering assisted immunochromatographic assay was developed for the detection of silk residues in artifacts.
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Novel green-emissive carbon dots as a dual-purpose fluorometric tool for sensitive vismodegib detection and pharmacokinetic analysis

Anal. Methods, 2026, 18,1937-1947
DOI: 10.1039/D6AY00073H, Paper

Ali M. Alaseem, Razan Orfali, Glowi Alasiri, Ramadan Ali, Al-Montaser Bellah H. Ali, Mohamed M. El-Wekil
Accurate determination of vismodegib (VISMO), a Hedgehog pathway inhibitor approved for the management of basal cell carcinoma and other malignancies, is essential for pharmacokinetic assessment, dose optimization, and therapeutic drug monitoring.
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An aptamer-CRISPR/Cas12a biosensor for rapid and sensitive detection of florfenicol

Anal. Methods, 2026, 18,1910-1916
DOI: 10.1039/D5AY01903F, Paper

Yufei Huang, Zhongling Zhao, Jianghao Li, Xinyu Wang, Hai Qu, Yehuan Zheng
A novel biosensing platform is developed for the rapid and sensitive detection of florfenicol by integrating aptamer-based molecular recognition with CRISPR/Cas12a-mediated trans-cleavage activity.
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Spectroscopic characterization of chiral substances: proline and glutamine

Anal. Methods, 2026, 18,1891-1900
DOI: 10.1039/D5AY02127H, Paper

Huiyu Yang, Xinrui Zhang, Qifubo Geng, Yang Gao, Zhuang Peng, Bo Su, Hailin Cui, Shengbo Zhang, Cunlin Zhang, Kai Li
Chiral substances such as proline and glutamine have garnered increasing attention due to their distinct biological roles in different enantiomeric forms.
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A CRISPR/Cas9-regulated dual-ring topological allosteric probe for detection of the EGFR L858R resistance mutation in CTCs

Anal. Methods, 2026, 18,1815-1825
DOI: 10.1039/D5AY02124C, Paper

Chenxing Wang, Dandan Li, Rongjun Yu, Jianjiang Xue, Wei Xie, Qiongyuan Zhang, Xiao Gui, Lilin Wang, Sihan Guo, Yu Xie, Yu Jiang, Gang Liu, Jiangling Wu
A CRISPR/Cas9-regulated dual-ring topological allosteric probe was developed for ultrasensitive and specific detection of the EGFR L858R mutation, where the released reporter ring triggers RCA and fluorescent probe hybridization for signal readout.
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Chromaeus: a browser-based analytical platform for quantitative colorimetric measurement in digital chemistry applications

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00168H, Paper

Hichem Moulahoum, Faezeh Ghorbanizamani
A standalone browser-based analytical platform standardizes digital colorimetric measurements through quantitative calibration, validation metrics, and instrument-free analysis.
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Investigating the reproducibility and repeatability of commercial SERS substrates using a new methodological approach

Anal. Methods, 2026, 18,1917-1927
DOI: 10.1039/D6AY00022C, Paper

Qiqian Liu, Aicha Azziz, Vlad Cucuiet, Marjan Majdinasab, Celia Arib, Xiang Yang, Weiling Fu, Monica Focsan, Frédéric Amiard, Mathieu Edely, Marc Lamy de la Chapelle
Standard protocol to evaluate the SERS performances. Highly reproducibility at the local scale/poor reproducibility at the large scale. Highly repeatability at the large scale/poor repeatability at the local scale.
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Calorific value measurement of large-particle coal using NIRS–XRF fusion spectroscopy and nonlinear residual correction: a PLS–AE–RR model framework

Anal. Methods, 2026, 18,1881-1890
DOI: 10.1039/D5AY01967B, Paper

Yifan Qiao, Ruonan Liu, Bin Li, Rui Gao, Jiaxuan Li, Lei Zhang
This study addresses the on-site challenge posed by large-particle (0–6 mm) coal samples, which are difficult to mill and exhibit pronounced matrix effects and strong nonlinearities that degrade the accuracy of online calorific-value prediction.
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A Wi-Fi-enabled, automated device for pathogen detection via LAMP and paper-based colorimetric assay

Anal. Methods, 2026, 18,1851-1869
DOI: 10.1039/D5AY02131F, Paper

Yi Ting Lai, Nae Yoon Lee, Jyh Jian Chen
A Wi-Fi-enabled automated microfluidic device integrates DNA extraction, wax-triggered LAMP amplification, and paper-based colorimetric detection for rapid, portable identification of Vibrio vulnificus at the point of care.
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Electrochemical quantification of condensed tannins in extracts and herbal medicines using 3D-printed electrodes

Anal. Methods, 2026, 18,1841-1850
DOI: 10.1039/D5AY02048D, Paper

Dayane Vasconcelos Petine, Diego Godina Prado, Mariana Cardoso Marra, Raquel Gomes Rocha, Nélio Inácio Gravata Inoque, Mário Machado Martins, Robert D. Crapnell, Craig E. Banks, Alberto de Oliveira, Rodrigo Alejandro Abarza Munoz, Raquel Maria Ferreira Sousa
A 3D-printed carbon-based electrode within a 3D-printed cell can be used to quantify condensed tannins in extracts and herbal medicines after proper electrode surface treatment and sample preparation steps.
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Ehrlich chromogenic reaction-based merging-zone flow injection analysis for accurate and rapid determination of total indole phytohormones in plant resources

Anal. Methods, 2026, 18,1928-1936
DOI: 10.1039/D5AY01462J, Paper

Yan Zhang, Turghun Muhammad, Junxia Gao, Jia Zhao, Feiyu Zhan, Lianfeng Liu, Yinglian Chen
Automation, easy of operation, rapid detection, and low reagent and sample consumption make it an efficient and green analytical method for quantitative analysis of total indole phytohormones in plant extracts.
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Quantification and stability analysis of anthocyanins, chlorogenic acids and flavonoids in Roselle calyces

Anal. Methods, 2026, 18,1901-1909
DOI: 10.1039/D5AY02037A, Paper

Meng-Yao Hu, Dan Yang, Hai-Xia Xu, Yu-Xian Guo, Qing-Feng Zhang
An HPLC method for simultaneous analysis of anthocyanins, chlorogenic acids and flavonoids in Roselle calyces was developed.
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Fluorescence-based determination of cyclic adenosine monophosphate in nasal secretions of healthy and post COVID-19 patients using Tinopal CBS-X

Anal. Methods, 2026, 18,1870-1880
DOI: 10.1039/D5AY01820J, Paper

Mohamed S. Imam, Nouf F. M. Hashim, Sarah M. A. Alqarni, Shuruq M. A. Aljauid, Khalid M. R. AlGhazal, Ghaida M. Alruways, Bariah S. AlOtaibi, Reem A. R. Alhafi, Hissah F. M. Al dawood, Sherif M. Elaidy
A rapid fluorescence-based method using Tinopal CBS-X to quantify cAMP in human nasal secretions.
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Determination of thallium by solution anode glow discharge atomic emission spectrometry with an interference filter for spectral discrimination

Anal. Methods, 2026, 18,1826-1833
DOI: 10.1039/D6AY00031B, Paper

Jinmei Wang, Tao Hu, Peichao Zheng, Biyong Zhang, Xuanyu Luo, Junhao Xiang, Lianbo Guo
A novel, cost-effective solution anode glow discharge atomic emission spectrometry system with an interference filter (SAGD-IF-AES) is reported for the determination of trace thallium (Tl) in water.
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Versatile method for addressing the issue of methanol in distilled spirits using a compact near-infrared spectrometer

Anal. Methods, 2026, 18,1834-1840
DOI: 10.1039/D5AY02163D, Paper

Celio Pasquini, Maria C. Hespanhol
Direct analytical method based on the spectral absorption characteristics of the third overtone (1100–1280 nm) of the near-infrared region to determine methanol.
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Random forest models accurately classify synthetic opioids using high-dimensionality mass spectrometry datasets

Anal. Methods, 2026, 18,1804-1814
DOI: 10.1039/D5AY01677K, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Kourosh Arasteh, Steven Magana-Zook, Colin V. Ponce, Roald Leif, Alex Vu, Mark Dreyer, Brian P. Mayer, Audrey M. Williams, Carolyn L. Fisher
Detection of novel threat agents presents several challenges, a principle one being the development of untargeted methods to screen an increasing number of threat chemicals whose exact structures are unknown.
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A fluorogenic and ultrabright probe for imaging of lipid droplets in live and fixed cells

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00188B, Paper

Guorui Zhao, Guanchang Ji, Dongdong Song, Yarong Ma, Guanzheng Tian, Guizhen Zhang
Lipid droplets(LDs) play an vital role in energy homeostasis and cellular signaling. Therefore, the visualization of LDs is very important. To this end, a new LD-specific fluorescent probe, TPE-Q, was...
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A self-priming dual-hairpin probe-mediated primer exchange reaction for label-free fluorescence detection of microRNA in Alzheimer's disease

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02058A, Paper

Zhiqiang Liu, Zhaoxia Zang, Dawei Li, Shanshan Wu
The accurate detection of microRNA (miRNA) biomarkers associated with Alzheimer's disease (AD) is critical for early diagnosis and therapeutic monitoring.
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Light-Induced Self-Assembly of Ag NPs for Dual-Range Colorimetric Detection of Ethanol

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00015K, Paper

Jiahang Yu, Yimeng Huang, Ruihao Huang, Xiao Sun, Ke Zhao, Zongyou Ben
Rapid and accurate ethanol determination is crucial for quality control in the alcoholic beverage industry. We report a facile, green colorimetric sensor based on ethanol-modulated self-assembly of Ag NPs (Nano...
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Direct-inject suppressed ion chromatography-mass spectrometry method with online preconcentration for short- and ultra short-chain perfluoroalkyl carboxylic acids in fresh water

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY02105G, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Jessica Clouthier, Trevor C. Vandenboer, Cora Young
This work developed a novel analytical method to detect short- and ultra-short chain perfluoroalkyl carboxylic acids (PFCAs) in freshwater samples by direct injection using ion chromatography mass spectrometry (IC-MS). Ultra-short...
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Simultaneous determination of six microplastics in drinking water by pyrolysis-gas chromatography/mass spectrometry

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01978H, Paper

Mingming Yin, Jingli Fan, Gang Cheng, Qiaoying Chang, Bing Shao, Ruobin Bai
In our study, we have successfully established a method to simultaneously detect six key MPs in drinking water: PE, PP, PS, PET, PMMA and PVC, employing stainless steel membrane filtration to enrich the MPs, and using Py-GC/MS for analysis.
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Balancing sensitivity and environmental safety in fluorescent probe design: improved ethanol-thermal carbon dots enable a leap in mercury(II) detection and zebrafish-assessed safety

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01948F, Paper

Xixuan Chen, Lei Zheng, Rongzhen Ma, Phong H. N. Vo, Jiaxin Ni, Songjun Guo
First zebrafish-based safety-assessed Hg²⁺-sensing carbon dots with ultrahigh performance resolve the trade-off between sensitive detection and environmental safety.
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AFM-derived cytoskeletal mechanics reveal ethanol-induced injury and vitamin C-mediated repair in SH-SY5Y cells

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY02053K, Paper

Xingyue Wang, Baohua Jia, Zuobin Wang, Kaige Qu
Oxidative stress and cytoskeleton-associated remodeling have been widely implicated, yet most current approaches to assess the cytoskeleton-associated organization and mechanics rely on fluorescent labeling or electron microscopy, which are invasive...
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Rapid identification of Vibrio vulnificus by Raman spectroscopy

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02030A, Paper

Mya M. Hlaing, Mingyu Han, Michelle Dunn, Paul R. Stoddart, Sally L. McArthur
The autochthonous estuarine bacterium Vibrio vulnificus (V. vulnificus) is an opportunistic human pathogen acquired through consumption of raw shellfish or open wound infections.
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Menthol-based hydrophobic deep eutectic solvent for vortex-assisted liquid–liquid microextraction and capillary electrophoretic determination of bioactive compounds in licorice

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01974E, Paper

Chenjiang Jiang, Xiaoqi Liu, Yunfeng Yuan, Haodong Zhou, Jinhua Zhu, Dandan Niu, Xiuhua Liu
Novel menthol-based HDES enables VA-LLME-HPCE for licorice bioactives.
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Enantioselective recognition and detection of cysteine using a three-dimensional Mn1/3Zn2/3CO3-rGO/CNT heterostructure-based molecularly imprinted sensor

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01993A, Paper

Yu Rao, Qian Jian, Chenrui Tan, Xinyao Yu, Xinwu Jiang, Hao Dai, Jiangyu Zhang, Ling Wu, Dan Li, Zhong Cao
3D Mn_1/3Zn_2/3CO₃-rGO/CNT chiral MIP sensor enables ultrafast enantioselective detection of L/D-Cys via dynamic electrochemistry.
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Highly stable ECL sensor based on a self-supplied H2O2 probe and MOF nanozyme for ultrasensitive environmental mycotoxin ZEA detection

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00104A, Paper

Shangming Xia, Xiang Gao, Kangyuan Sun, Fei Liang, Wenbin Jiang, Yanqiu Leng
Zearalenone (ZEA), an environmental mycotoxin produced by Fusarium fungi, is widely present in grains and grain products.
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Lanthanide-doped luminescent lateral flow immunoassay for the ultrasensitive and quick detection of methyltestosterone in fish samples

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00063K, Paper

Hechen Lu, Yaohui Xue, Jinhua Li, Ming Ma, Qingwei Song, Sihua Qian, Jian Li, Yuhui Wang
A highly sensitive lateral flow immunoassay using ultrabright YVO₄:Eu as the label probe was designed for the on-site and highly sensitive detection of methyltestosterone (MT) in fishes.
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Rational design of a chitosan-coated cobalt ferrite nanozyme coupled with a molecularly imprinted polymer for trace analysis of buspirone in complex matrices: application in spiked human plasma and environmental water samples

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00091F, Paper

Veronia S. Nazim, Ghada M. El-Sayed, Sawsan M. Amer, Ahmed H. Nadim
A colorimetric sensor for sensitive and selective determination of buspirone in spiked human plasma and environmental water samples based on a chitosan-coated cobalt ferrite nanozyme with a polydopamine MIP.
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Integrated supercritical fluid extraction and online ICP-MS nano-gram determination of titanium(IV) using calixarene hydroxamic acid

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01960E, Paper

Devarshi Thaker, Bignesh Thakur, Nirav Pandya, Yadvendra Agrawal
A selective supercritical fluid extraction-ICP-MS method enables ultra-trace determination of Ti(IV) in complex matrices with high extraction efficiency, low detection limits, and effective matrix removal.
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Determination of Vidarabine Using Fluorescein Sodium Probe-Based resonance Rayleigh scattering

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00028B, Paper

Xinyu Yang, Meng Li, Na Yin, Nian Ding, Hong Yu, Jinyi Mao, Zhixun Yu, Qianqian Su
The establishment of novel methods for drug content determination provides additional options for pharmaceutical quality control. A new method for detecting Vidarabine (Ara-A) has been developed, utilizing the acidic anionic...
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An "off-on" fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00068A, Paper

Zhuozhuo Ren , Qiongdan Zhang, Yuxing Wang, Zhuoer Chen, Ziyi Zhou, Da Qian
The activity of apurinic/apyrimidinic endonuclease 1 (APE1) is a key biomarker in breast cancer. We report an "off-on" fluorescent nanosensor (OOFN) that combines fluorescence resonance energy transfer (FRET) with magnetic...
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Development and application of a tetra-ARMS PCR assay for detecting indel polymorphisms in the bovine PRNP gene

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02047F, Technical Note

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Ye Xu, Siyu Yang, Siling Ding, Ting Xu, Jian Ge, Weiming Xiao, Like Zhu, Feng Guan
Insertion/deletion (indel) polymorphisms in the promoter region (23 bp) and intron 1 (12 bp) of the bovine PRNP gene influence gene expression and susceptibility to bovine spongiform encephalopathy (BSE).
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Rapid in situ analysis of tobacco chemical composition via ultrasonic nebulization extraction coupled with atmospheric pressure photoionization mass spectrometry

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01923K, Paper

Bing Qian, Yonghua Hu, Qiong Wu, Hongxing Li, Chulian Su, Chengyuan Liu, Yang Pan, Bingjun Han
A rapid in situ analytical method combining ultrasonic nebulization extraction and atmospheric pressure photoionization mass spectrometry allows direct analysis of endogenous components in complex tobacco matrices.
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An aptamer sensor based on peroxidase-like AgNPs@MOF for SERS and colorimetric dual-mode detection of sulfadimethoxine

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02128F, Paper

Renjie Zhao, Zhanye Yang, Xin Sun, Guantong Liu, Shutong Zhang, Xinge Ju, Yuru Wang, Yuan Tian, Lan Ding, Daqian Song
An AgNPs@MOF bifunctional nanocomposite (combining peroxidase-like catalytic properties and surface-enhanced Raman activity substrate) was synthesized.
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A novel fluorescent probe for hydrogen sulfide imaging in living inflammatory models

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01252J, Paper

Xin-Ai Shi, Yu Jiang, Pei Tang, Xin-Ru Zhang, Ge Wang, Hao Peng, Peng-Peng Wang, Li-Sheng Xu, Qian-Qian Han, Ya-Xi Ye
The chemical structure and schematic illustration of DHM-H2S for imaging H₂S in inflammatory models.
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A potential-resolved electrochemiluminescent aptasensor for simultaneously detecting MUC1 and HER2 on breast cancer exosomes

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02083B, Paper

Dong Jiang, Rui Li, Qingjiang Wang
A potential-resolved ECL aptasensor on a PDMS-patterned ITO electrode was developed for the simultaneous detection of MUC1 and HER2 proteins on breast cancer cell-derived exosomes, achieving reliable discrimination of exosomal subtypes.
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Synthesis of a hydrophilic covalent-organic framework@silica composites via oxidation reaction for mixed-mode liquid chromatographic separation

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00067C, Paper

Xingyun Zhao, Jiangyan Jin, Peng Wang, Yonghao Chai, Kairui Zhu, Liyun Zhang, Ruifang Wang, Ke Li, Rongfang Wu
Specifically, the oxidation of imine linked TAPT-TA-COF@SiO2 was prepared using 2,4,6-tris (4-aminophenyl-1,3,5-thiazine (TAPT) and 1,4-benzenedicarboxaldehyde (TA) as monomers through one pot cascade polymerization reaction on the surface of silica spheres,...
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Scanning ion conductance microscopy for living cell nanomechanics: principles, advances and applications

Anal. Methods, 2026, 18,1583-1593
DOI: 10.1039/D5AY01889G, Critical Review

Alexei A. Nastenko, Vasilii S. Kolmogorov, Alexander N. Vaneev, Peter V. Gorelkin, Natalia L. Klyachko, Alexander S. Erofeev
Scanning ion conductance microscopy provides unique insight into mechanical properties of living cells. It has found applications in drug screening and studying cancer, blood, neuronal and muscle cells and organoids.
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Research on spectroscopic determination and classification of diffuse reflectance for low-altitude UAV fuselage materials

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02162F, Paper

Dongliang Li, Yangyang Hua, Tingting Wang, Yangming Cao, Jianguo Liu, Hongxing Cai
A diffuse reflectance spectral database of 15 UAV materials was established. The KNN algorithm achieved perfect classification, and feature importance analysis identified key bands for targeted multispectral sensing.
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Harnessing CRISPR/Cas systems for food safety detection: biosensor design and emerging applications for food safety detection

Anal. Methods, 2026, 18,1559-1582
DOI: 10.1039/D5AY01791B, Critical Review

Yunwei Niu, Siyuan Wu, Jing Su
CRISPR Cas-based biosensors for food safety—Cas9, Cas12, Cas13a and Cas14 enable programmable recognition and readouts for pathogens, GMOs, food fraud, and chemical hazards (toxins, metals, antibiotics, pesticides).
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Biomass carbon quantum dots and Zr-MOFs as ratiometric fluorescent probes for sensitive detection of biogenic amines in shrimp samples

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01937K, Paper

Long Jing, Zheng Liu, Mengyu Su, Qirong Lu, Xiangfei Kong, Xiaoyan Wang, Guo-Cheng Han
Dual-response-reverse ratiometric fluorescence paper sensor based on G-BCQDs/Zr-MOFs for biogenic amine (spermine) detection via smartphone RGB analysis.
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Validation of an RP-HPLC method for simultaneously quantifying three UV filters encapsulated into nanostructured lipid carriers

Anal. Methods, 2026, 18,1732-1743
DOI: 10.1039/D5AY01558H, Paper

Andressa C. Schneid, Hatylas Azevedo, Margarete M. de Araújo
Development and validation of an accurate and precise analytical method able to determine UV-filter content, based on the extraction method, and encapsulation efficiency, supporting formulation optimization and industrial pipeline.
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A hydrophilic interaction UPLC-MS/MS quantitative method for the quantification of saracatinib in the human liver microsome matrix and its application in in vitro metabolic stability assessment

Anal. Methods, 2026, 18,1695-1708
DOI: 10.1039/D5AY02096D, Paper

Mohamed W. Attwa, Adnan A. Kadi
The hydrophilic interaction UPLC-MS/MS method was used for the quantification of saracatinib in HLMs. Small changes to the N-methyl piperazine in drug design might improve the metabolic stability of new derivatives compared with that of saracatinib.
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A molecularly imprinted electrochemical sensor based on dual functional monomers for selective determination of nimodipine

Anal. Methods, 2026, 18,1674-1684
DOI: 10.1039/D5AY01486G, Paper

Ying Wang, Xuyuan Sun, Minmin Liu, Zhengyuan Dai, Xiaoyu Yang, Li Li, Yaping Ding
A molecularly imprinted electrochemical sensor (MIECS) for the detection of nimodipine (NMD). The response of the MIECS to NMD showed a linear relationship from 10⁻¹⁴ M to 10⁻⁸ M, with a detection limit of 2.97 × 10⁻¹⁵ M.
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Comparison of biological materials to assess their suitability for Raman spectroscopic detection of chronic myeloid leukemia

Anal. Methods, 2026, 18,1654-1666
DOI: 10.1039/D5AY01729G, Paper

Sreeparna Nath, Arti Hole, Bhausaheb Bagal, Hemanth Noothalapati, Rukmini Govekar, C. Murali Krishna
Raman spectroscopic analysis of blood cells, plasma and serum discriminates CML from healthy samples. Spectral evaluation and data fusion of biofluids and cells demonstrate potential of Raman spectroscopy for future clinical CML studies.
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A pyrene-derived fluorescence probe based on π–π stacking and excimer formation for DNA detection and intracellular visualization

Anal. Methods, 2026, 18,1751-1756
DOI: 10.1039/D5AY02071A, Paper

Songtao Cai, Chuanyan Zhou, Caixia Xue, Yanfang Huo, Chang Liu, Yingying Jing, Yan Fan, Yanxia Zhang, Xianshun Zeng
A pyrene-derived fluorescent probe L with high selectivity and sensitivity for DNA detection and intracellular visualization via π–π stacking and excimer formation has been developed.
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Expanding luminol activity: UV-enhanced peroxidase-like activity for dual-mode wide-pH biosensing

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02106E, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Mohamed Hassan Mahana, Syed Rahin Ahmed, Poushali Das, Seshasai Srinivasan, Amin Reza Rajabzadeh
A microfluidic device is composed of two channels, one channel for the peroxidase reaction, which contains luminol and TMB, while the second channel for the chemiluminescent reaction, which contains luminol and a catalyst like iron salt.
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A novel approach for discriminating lipophilic bisphosphonate-based pharmaceuticals using a potentiometric array

Anal. Methods, 2026, 18,1626-1636
DOI: 10.1039/D5AY01639H, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Tatiana V. Shishkanova, Annemarie Skálová-Coufal, Jaroslav Otta, Martin Člupek, Martin Vrňata
Evaluation of lipophilicity of bisphosphonate-based pharmaceuticals using a potentiometric array.
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Pyrene-based fluorescent molecularly imprinted polymer for the rapid and selective detection of oxytetracycline based on the inner filter effect

Anal. Methods, 2026, 18,1667-1673
DOI: 10.1039/D5AY02167G, Paper

Tianyi Zhu, Jian Zhang, Danyi He, Yiwen Yang, Hailong Wang, Jianbo Wang, Longhua Guo, Lei Li, Jianrong Chen, Yanbo Zeng
Synthesis process for the Al-Py monomer, preparation of Py-FMIP and the procedure for oxytetracycline (OTC) detection using Py-FMIP.
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A dual-mode biosensor for alkaline phosphatase detection based on DCIP and Eu3+-calcein

Anal. Methods, 2026, 18,1744-1750
DOI: 10.1039/D5AY01977J, Paper

Bingyu Fu, Yanru Shao, Zhixiong Zheng, Shengxin Wen, Yingli Guo, Qi Xu, Hualin Yang
A dual-mode ALP biosensor was designed. ALP catalyzed the dephosphorylation of AAP to form AA and PO₄³⁻. Then, AA reduced DCIP from blue to colorless, while PO₄³⁻ chelates with Eu³⁺ to restore Eu³⁺-calcein fluorescence.
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A Lab-on-a-Drone for turbidimetric and nephelometric analysis of environmental water samples with real-time data transmission

Anal. Methods, 2026, 18,1709-1719
DOI: 10.1039/D5AY01927C, Paper

Jackson Rodrigo dos Santos Silva, Vinícius de Alcântara Carvalho, Gabriel Bercley de Lima Vitorino, João Paulo Barbosa de Almeida, Aldebarã Fausto Ferreira, Willian Toito Suarez, Elton de Lima Borges, Vagner Bezerra dos Santos
A Lab-on-a-Drone platform for real-time water analysis with data transmission via Wi-Fi based on a turbidimeter/nephelometer coupled to an unmanned aerial vehicle (UAV).
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A multifunctional core–shell CoFe2O4@ZIF-67 nanocomposite for simultaneous detection and removal of Pb2+ in water

Anal. Methods, 2026, 18,1720-1731
DOI: 10.1039/D5AY01896J, Paper

Yingying Wang, Tengda Zhao, Wei Yin, Zhuoxun Liu, Shijuan Tian, Li zhang, Sha Sha, Hongling Zhou, Huanbao Fa, Hang Li, Gang Li, Yuqin Xu
To address the challenge of integrated detection and removal of toxic Pb²⁺ from water, a novel magnetic core–shell nanocomposite, CoFe₂O₄@ZIF-67, was developed.
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A coumarin functionalized NIR fluorescent probe based on the thiopyrone skeleton for the detection of Cys and its applications

Anal. Methods, 2026, 18,1601-1609
DOI: 10.1039/D5AY02068A, Paper

Huan Zhang, Baoze Guo, Junqing Zhou, Cong Sun, Jinwei Zhang, Shuai Guo, Songhua Zhu, Youlai Zhang
A novel near-infrared fluorescent probe, PH-Cys, was constructed on a thiopyrone scaffold for the selective detection of cysteine. It exhibits a large Stokes shift (217 nm), high sensitivity (LOD: 13.60 nM), and a rapid response (3.0 min).
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Multi-detector frit-inlet asymmetric flow field-flow fractionation method development for nanoparticle mixtures: deeper analysis beyond ISO quality standards

Anal. Methods, 2026, 18,1637-1653
DOI: 10.1039/D5AY01758K, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Rand Abdulrahman, Panida Punnabhum, Lisa Van Den Driest, Nicholas J. W. Rattray, Robin Capomaccio, Kevin Treacher, Yvonne Perrie, Zahra Rattray
This study presents the development of frit-inlet asymmetric flow field-flow fractionation for analysing heterogeneous nanoparticle mixtures, introducing performance metrics beyond current ISO standards.
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A novel cloud point extraction method using a benzimidazole-based ligand for the preconcentration of toxic heavy metals in water and food samples

Anal. Methods, 2026, 18,1610-1617
DOI: 10.1039/D6AY00036C, Paper

Olcay Ozdemir, Celal Duran, Emre Mentese, Duygu Ozdes
In the present research, a novel CPE method was developed for the separation and preconcentration of toxic Cu(II), Ni(II), Pb(II), and Cd(II) ions in environmental water and vegetable samples, prior to their determination by FAAS.
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Monitoring production processes of pharmaceuticals and farm chemicals with near-infrared spectroscopy coupled with the interaction moving window partial least squares method

Anal. Methods, 2026, 18,1618-1625
DOI: 10.1039/D5AY01996F, Paper

Shuming Lan, Min Ding, Honghong Wang, Yan Zhang, Yiping Du
The novel iMWPLS algorithm optimally selects NIR spectral intervals, boosting PLS model accuracy for online process monitoring. It offers a robust, interpretable tool for NIR-based quality control in pharmaceuticals.
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Ultrasensitive toltrazuril sulfone detection in eggs via a AuNPs/NH2–Ce-MOF-enhanced electrochemical immunosensor

Anal. Methods, 2026, 18,1685-1694
DOI: 10.1039/D5AY01781E, Paper

Jinghan Li, Kemian Liu, Xiaoge Duan, Huajuan Mo, Siyu Liu, Shengda Qin, Liang Luo, Yu Wen, Qiuhua Wang, Hailan Chen
Schematic illustration of the fabrication and detection mechanism of the GCE/NH₂–Ce-MOF/AuNPs electrochemical immunosensor for TOS quantification.
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Characterizing noninvasively conservation status of historical wet collections using spatially offset Raman spectroscopy

Anal. Methods, 2026, 18,1594-1600
DOI: 10.1039/D5AY01654A, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Wren Montgomery, Chelsea McKibbin, Ana Blanco, Sam Walker, James Maclaine, Roberto Portela Miguez, Patrick Campbell, Robert Stokes, Pavel Matousek, Sara Mosca
Fluid-preserved specimens are central to the scientific and cultural value of natural history collections, yet their conservation is challenged by chemical and physical instabilities of both the specimens and their preservation media.
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Quantification of nitrite in beverages and pickled foods with a high-sensitivity amperometric biosensor enhanced by a multilayer perceptron neural network

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01922B, Paper

Zhongwei Liang, Guang Yang, Zidong Chen, Bingxin Zhong, Bin Ran, Bo Liu, Jialin Liang, Teng Shen, Peng Liu, Daqi Chen, Chaozhan Chen
An MLP-enhanced amperometric biosensor enables high-sensitivity quantification of nitrite in beverage and pickled food matrices.
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A multimodal approach integrating spectroscopy, deep learning guided molecular docking, and molecular dynamics simulation for predictive assessment of pioglitazone to albumin binding for formulation development

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01534K, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Saswata Banerjee, Sk. Abdul Amin, Shovanlal Gayen, Sakshi Priya, Yashika Tomar, Rajeev Taliyan, Gautam Singhvi
A multimodal approach to analyse human serum albumin and pioglitazone interaction and deduce the feasibility of a formulation.
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The site-directed modification of nanobodies through a genetic engineering technology for the fluorescent test strip detection of fenpropathrin

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00089D, Paper

Yu Zhang, Luo-Ji Yang, Hui Zhang, Jin-Feng Zhang, Xiao-Ming Liang, Jin-Zhi Han, Aori Qileng, Zhen-Lin Xu
The lateral flow immunochromatography assay (LFIA) offers a rapid and on-site method for food safety.
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Use of ATR-FTIR with D/H Substitution for Assessing Water Uptake in Alkali Perchlorate Matrices

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY02133B, Paper

Mohammed Alfatih Hamid, Ezgi Kutbay, Sefik Suzer
Use of FTIR spectroscopy together with isotopic exchange offers a powerful approach towards understanding cation dependence of water uptake in alkali perchlorate salts at room temperature. ATR-FTIR data reveal distinct...
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Bisbenzimidazoles with Benzenediyl Spacer: An Efficient Fluorophore for Dihydrogenphosphate Sensing and Targeted Living Cell Imaging

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY02020D, Paper

Bhaskar Biswas, Rakesh Debnath, Subhajit Saha, Subhankar Kundu, Abhranil De, Sourav Mondal, Muddukrishnaiah Kotakonda, Nilanjan Dey
A bisbenzimidazole-based fluorescent probe (PBZ) has been developed for the selective detection of dihydrogen phosphate (H₂PO₄⁻) ions in a CH₃CN–water (7:3, v/v) mixed solvent system. The probe exhibited pronounced spectral...
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Precision Traceability of Trace Psychoactive Substances under Water Quality Parameter Perturbations in Complex Matrix Situation

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY01865J, Paper

Zheng-Jie Han, Dahai Zhang, Meng-Yue Fang, Yan Zhang, Dao-Ling Wang, Jie Hou, Hao-Chen Yan, Kai-Xuan Luo, Xianguo Li
Wastewater-based drug testing using LC–MS/MS is widely applied to monitor the use of psychoactive substances (PSs), which are chemicals that act on the central nervous system and alter perception, mood,...
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An on-tissue chemical derivatization method for MALDI-MSI analysis of anthraquinones in mouse tissues

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY01880C, Paper

Yong-An Yan, Siqi Han, Pian Jin, Xiao-Bo Zhao, Yan-Ping Shi, Wei Ha
Acquisition of tissue spatial distribution information is essential for identifying sites of action, assessing pharmacological effects and elucidating mechanisms, thereby providing evidence for drug development. Anthraquinones, including rhein, emodin, aloe-emodin,...
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Whole-Cell SERS Fingerprints for Rapid Toxicant Attribution in Water

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00054A, Paper

Ziyang Weng, Ying Zhou, Nan Ma, Meikun Fan
Effect-based bioassays provide rapid warning of adverse impact but rarely identify the causative stressor, whereas targeted chemical analysis identifies selected pollutants yet offers limited toxicity information, particularly for mixtures. Here,...
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A new method for quantitation of cyanuric acid in water based on image analysis of drying patterns using computer vision

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02122G, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Neta Caspin, Olga Mindlina, Uriah H. Sharon, Vladimir V. Gridin, Maor Manevich, Israel Schechter
Coffee ring effect and computer vision used for quantitation of cyanuric acid in water.
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Cas13a/crRNA trans-cleavage triggered primer exchange reaction based self-priming chain extension for sensitive and label-free infantile pneumonia related microRNA analysis

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00090H, Paper

Jia Yang, Qiuling Huang
Accurate and sensitive detection of microRNA (miRNA) is crucial for both pathophysiological studies and clinical diagnostics. Conventional amplification methods often face limitations such as dependence on thermal cycling, susceptibility to...
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A novel ratiometric electrochemical aptasensor based on β-cyclodextrinylated graphene and Fc/β-CD host–guest recognition for ultrasensitive detection of zearalenone

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00074F, Paper

Yao Tian, Mengsi Liang, Yongdong Wei, Yuxin Wang, Ningbo Li, Yaoyong Wang
A ratiometric electrochemical aptasensor has been successfully developed for ultrasensitive detection of zearalenone. Furthermore, this method has been successfully applied to detect ZEN in wine.
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Construction of Near-Infrared Fluorescent Probe for Cysteine and Its Application in Oxidative Stress Research

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00075D, Paper

Lixia Zhang, Xingxing Zhao, Mei Yang, Siyu Yan, Xiaoxing Li, Jian Su, Hongmei Liu
Cysteine (Cys) is a key endogenous thiol in the body, and its active sulfhydryl group plays a core role in physiological processes such as glutathione synthesis, antioxidation, protein disulfide bond...
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Microwave-assisted hydrolysis for the physicochemical characterization of functional methacrylic polymers and their bioconjugates

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00012F, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Ilaria Porello, Paola Nastri, Marta Bozzi, Filippo Moncalvo, Philippe Gonzalez, Alessandro Sacchetti, Francesco Cellesi
This study presents microwave-assisted hydrolysis (MAH) strategy for precise molar mass determination of functional methacrylic polymers and their protein conjugates prepared by controlled/living polymerization, enabling fast, reliable characterization of complex biohybrid materials.
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Introduction to laser cleaning in cultural heritage

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY90017H, AMC Technical Brief

Analytical Methods Committee, AMCTB No. 121
Laser cleaning provides a precise and effective method for removing unwanted contaminants from cultural heritage objects and buildings, and is increasingly commonly used in conservation of delicate surfaces, providing an alternative to the use of solvents or abrasives.
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UiO-66-NH2@benzotrithiophene-based COF composites with core–shell structure for effective adsorption of dyes

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00027D, Paper

Xinhong Zhao, Rong Ma, Yuanyuan Li, Yulong Ma, Wenxin Ji, Yonggang Sun
Possible mechanism of dye adsorption on UiO-66-NH₂@BTT–BZ.
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Portable “dual-signal-on” colorimetric and photothermal biosensor for accurate detection of Hg2+ based on split Cas12a-mediated cascade strand displacement and phosphorothioate-modified G-quadruplex DNAzyme

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01924A, Paper

Yi Tian, Jiaxuan Chen, Fengzheng Chen, Yu Ling, Shengyue Zheng, Daizhen Jian, Qiong Tang, Song Wang, Kai Shi
By integrating an SCas12a-mediated cascade strand displacement reaction with a phosphorothioate-modified G-quadruplex reporter, a portable “turn-on” colorimetric/photothermal system was engineered for sensitive detection of Hg²⁺.
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PVA/AgNPs hydrogel SERS substrate combined with machine learning for highly sensitive detection of organic selenium species

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY02045J, Paper

Huaqing Ling, zhixi Zhao, Ziqi Zhang, Shan Chen, Zulihaya Maimaiti
Accurate detection of organic selenium speciation in soil and water was essential for preserving ecological safety and protecting human health. The performance of a surface-enhanced Raman scattering (SERS) sensor was...
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Optimization of aptamer-triggered hybridization chain reaction for rapid visual ATP detection using gold nanoparticles

Anal. Methods, 2026, 18,1541-1547
DOI: 10.1039/D5AY01738F, Technical Note

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Shengli Zhou, Hiroko Fukaya, Shunsuke Watanuki, Wei Liu, Maasa Yokomori, Muneyuki Matsuo, Kazuya Okada, Yukina Yoshioka, Keitaro Yoshimoto
The development of fast, simple, and visual methods for detecting adenosine triphosphate (ATP) is crucial for point-of-care diagnostics and environmental monitoring.
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Synergistic electrocatalysis in a Ag2S@MoS2 nanocomposite enables an ultrasensitive label-free immunosensor of prostate specific antigen

Anal. Methods, 2026, 18,1513-1519
DOI: 10.1039/D5AY01955A, Paper

Yuxue Dai, Di Wang, Yu Shi, Shuangyu Zhang, Shuqi Zhang, Jiayue Wang, Dayong Song, Xiaowen Wu
This sensor utilizes a synergistic Ag₂S@MoS₂ nanocomposite, which combines the large surface area of MoS₂ with the high conductivity of Ag₂S, enabling ultra-sensitive PSA detection down to 0.17 pg mL⁻¹ and reliable accuracy in serum analysis.
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A candidate reference measurement procedure for aldosterone measurement in human serum and urine on the basis of isotope dilution liquid chromatography-tandem mass spectrometry

Anal. Methods, 2026, 18,1492-1502
DOI: 10.1039/D5AY02023A, Paper

Siyu Chen, Junyi Wu, Zihan Liu, Yuchen Wan, Chuanfen Xie, Yingguo Wang, Zechen Liu, Ke Chen, Jiashu Yang, Xinyi Rao, Hui Yuan
A candidate reference LC-MS/MS method was developed and validated for quantifying aldosterone in serum and 24 h urine. The method shows high accuracy and traceability, supporting the standardization of aldosterone measurements.
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DARA-Net: a dual-path residual attention network for denoising AFM images under complex noise conditions

Anal. Methods, 2026, 18,1479-1491
DOI: 10.1039/D5AY01701G, Paper

Tenglong Yu, Yuxi Huang, Quan Gu, Hongkun Li, Shibo Liu, Qiuyang Deng, Zuobin Wang
A GAN-based content enhancement framework is proposed for denoising AFM images under complex noise conditions, enabling effective noise suppression while preserving fine morphological features.
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Precision detection of rifampicin-resistant rpoB_L378R mutation in Mycobacterium tuberculosis with CRISPR-Cas12a

Anal. Methods, 2026, 18,1442-1453
DOI: 10.1039/D5AY01718A, Paper

Yuma Yang, Li Yang, Hao Ma, Shuming Zhang, Yue Zhu, Sihan Zhang, Xiting Lin, Haitao La, Xiumin Gu, Jing Ma, Sihao Zhao, Yinfeng Yang, Hetian Lei, Yanhui Yang
Novel RPA-CRISPR-Cas12a assay detects the rifampicin-resistant rpoB_L378R mutation.
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Solvent-mediated probe ultrasonication (SM-PUS) approach for the effective synthesis of metal/metal oxide nanosheets under ambient conditions

Anal. Methods, 2026, 18,1422-1432
DOI: 10.1039/D5AY01845E, Paper

Manusha Dissanayake, Maheshika Kumarihamy, Hui-Fen Wu
Schematic illustration of solvent-mediated probe ultrasonication for the synthesis of 2D metal nanosheets under ambient conditions.
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Discrimination between domestic and imported red chili powders using laser-induced breakdown spectroscopy: variable selection and comparative evaluation of machine learning algorithms

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01852H, Paper

Hyemin Jung, Sandeep Kumar, Yujin Oh, Sang-Ho Nam, Yonghoon Lee
This study demonstrates that laser-induced breakdown spectroscopy, combined with systematic variable selection and machine learning, enables efficient discrimination between domestically produced and imported red chili powders in Korea.
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Nitrogen/sulfur co-doped blue fluorescent carbon dots as a bifunctional probe for highly sensitive detection of chlortetracycline and lead ions

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00040A, Paper

Yue Liu, Yanbo Fu, Sixuan Li, JingHu, Shaogui Wu
Blue fluorescent carbon dots (NS-CDs) synthesized by oxalic acid and 1-amino-2-naphthol-4-sulfonic acid were successfully used for simultaneous monitoring of chlortetracycline (CTC) and lead ions (Pb²⁺) in food and environment.
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AlphaLICA-based homogeneous immunoassay for highly sensitive detection of streptomycin in infant formula

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02160J, Paper

Dian Li, Shangbin Kao, Mengwei Li, Yuan Qin, Xiumei Zhou, Xueqin Zhao, Jie Chen, Biao Huang
Addressing the need for infant food safety, this homogeneous AlphaLICA assay enables highly sensitive detection of streptomycin residues in infant formula within 15 minutes, providing a powerful tool for high-throughput screening.
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Single-step duplex CRISPR coupled with lateral flow assay for point-of-care detection of human immunodeficiency virus and Treponema pallidum

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02168E, Paper

Ziwen Xu, Muyun Wei, Mingming Jiang, Yunxiang Wang, Kunlun He
A single-step duplex CRISPR assay that integrated parallelized Cas12a and Cas13a in one-pot, coupled with visual lateral flow assay, enabling simultaneous point-of-care diagnosis of human immunodeficiency virus and Treponema pallidum.
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Dynamic multiple reaction monitoring for high throughput detection and quantitation of polycyclic aromatic compounds

Anal. Methods, 2026, 18,1503-1512
DOI: 10.1039/D5AY01950H, Paper

Zhe Xia, Thor Halldorson, Nipuni Vitharana, Marcus Kim, Dami Daramola, Chris Marvin, Philippe J. Thomas, Reyd A. Dupuis-Smith, Jennifer F. Provencher, Gregg T. Tomy
This study presents a dynamic MRM method for the simultaneous analysis of 122 polycyclic aromatic compounds, including PAHs, alkylated PAHs, halogenated PAHs, heterocyclic PACs, and halogenated HPACs using gas chromatography-tandem mass spectrometry.
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Single-cell mass cytometry reveals distinct immune signatures in colorectal cancer

Anal. Methods, 2026, 18,1520-1529
DOI: 10.1039/D5AY01928A, Paper

Yu Yang, Botao Wang, Jing Xun, Shuyi Zhang, Lei Yang, Yuan Ma, Junhong Ma, Xiangyang Yu, Yan Wang, Lanqiu Zhang, Qi Zhang, Ximo Wang
By leveraging the high-parameter analytical capacity of mass cytometry, samples from healthy donors, adenoma patients and colorectal cancer patients were analyzed to identify disease-specific immune signatures.
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A polyaniline-based ECL cytosensor for sensitive detection of circulating tumor cells

Anal. Methods, 2026, 18,1406-1411
DOI: 10.1039/D5AY02146D, Paper

Yongqi Zhang, Tiantong Wu, Chen Yan, Meixing Li, Qingming Shen
A sensitive ECL cytosensor based on polyaniline and the HCR amplification strategy for detection of circulating tumor cells.
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A visual protocol for sensitive Vibrio parahaemolyticus detection based on hybridization chain reaction cascaded DNAzyme

Anal. Methods, 2026, 18,1433-1441
DOI: 10.1039/D5AY02046H, Paper

Jiali Wang, Qian Cui, Shu Zeng, Danfeng Fu, Daoyun Peng, Fei Chen, Yi Wan, Anwei Wang
Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most harmful pathogenic bacteria derived from seafood, and it can cause great damage to aquaculture and human health, even at low concentrations.
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CRISPR/Cas12a platform activated by a protospacer adjacent motif-engineered DNA circuit for specific target sensing

Anal. Methods, 2026, 18,1397-1405
DOI: 10.1039/D5AY02079D, Paper

Rui Wang, Jiayue Li, Yuchen Zheng, Ning Wei, Wei Li
This study presents the development of a CRISPR/Cas12a platform. Sensitive and selective detection of specific targets is achieved. Moreover, trace amounts of these targets were successfully detected in real samples.
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Aptamer-functionalized 3D gold nanodendrite platform for electrochemical detection of circulating tumor cells in breast cancer

Anal. Methods, 2026, 18,1412-1421
DOI: 10.1039/D5AY01680K, Paper

Ruochen Wu, Li Lu, He Jiang, Dali Hu, Hepeng Su
This study introduces an advanced electrochemical biosensor utilizing 3D gold nanodendrites (AuNDs) functionalized with dual aptamers to achieve highly sensitive detection of circulating tumor cells in breast cancer.
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A MOF with high specific surface area for rapid separation and determination of β-adrenergic receptor blockers in pork by open-tubular capillary electrochromatography

Anal. Methods, 2026, 18,1454-1462
DOI: 10.1039/D5AY01941A, Paper

Lidi Gao, Jiawen Yu, Dongsheng Zhao, Xinyu Liang, Jun Wang, Fuquan Zhao, Ming Zhao, Hongtao Chu, Shili Qin
A simple, rapid and reliable method for detection of three β-adrenoceptor blockers in pork was established on an NH₂-MIL-125 OT column.
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Dual-organelle-targeted near-infrared carbon dots for visualizing glucose dynamics in cellular energy metabolism with potential in exercise physiology

Anal. Methods, 2026, 18,1472-1478
DOI: 10.1039/D5AY01904D, Paper

XiaoWen Duan, XiaoDong Cheng
B-OH-CDs, a dual mitochondria-lysosome targeted NIR probe, was developed for non-enzymatic glucose detection. It enables real-time visualization of intracellular glucose dynamics and serum quantification for probing energy metabolism.
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A novel 3D-CB/HEC–CH@Cu hybrid electrode for sensitive and rapid ammonia nitrogen detection in environmental water

Anal. Methods, 2026, 18,1530-1540
DOI: 10.1039/D5AY01860A, Paper

Pablo Henrique Silva Martins, Hugo Leonardo Souza Lara Leão, Polyana Fernandes Pereira
A low-cost 3D-printed carbon black electrochemical electrode modified with an HEC/chitosan bilayer and copper enables sensitive, accurate, and sustainable determination of ammonia nitrogen in water.
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Novel low-rank coal-based CDs for the detection of Fe3+

Anal. Methods, 2026, 18,1463-1471
DOI: 10.1039/D5AY01773D, Paper

Wenwen Wu, Xiang Han, Siyu Zhang, Lele Li, Meili Du, Fuxin Chen
In this study, a novel type of coal-based carbon dots (CDs) was synthesized using Inner Mongolia lignite as the carbon source and H₂O₂ as the oxidizing agent, and it was successfully applied for the detection of Fe³⁺ ions.
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