RSC - Anal. Methods latest articles

Quantitative determination of maslinic acid and oleanolic acid in olive pomace using high-speed shear extraction and liquid chromatography

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY01925G, Paper

Sheng-Bing Wang, Kun Yang, Dong Pei, Lu-Mei Pu, Xinyi Huang
Olive pomace contains abundant maslinic acid and oleanolic acid, maslinic acid and oleanolic acid are pentacyclic triterpenoid compounds with excellent biological activity. In this study, a method combining high-speed shear...
The content of this RSS Feed (c) The Royal Society of Chemistry

Correction: Rapid point-of-care detection of vitamin D deficiency using disposable electrochemical immunosensors based on a La2O3-WO3@CNT Bi-metal oxide nanocomposite

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY90044E, Correction

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Omar Ramadan, Mostafa Ahmed, Pradeep Kumar Brahman, Daohong Zhang, Rabeay Y. A. Hassan
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Imaging of individual concanavalin a proteins by atomic force microscopy

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02036K, Paper

Jiani Li, Jing Hu, Bowei Wang, Ying Wang, Jingran Zhang, Yujuan Chen, Zuobin Wang
Determining the monomolecular structure of lectins, such as concanavalin A (Con A), is crucial for understanding immune recognition mechanisms.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Enzyme-Free and Product-Accelerated DNA Walker Cascade for Ultrasensitive Detection of Tic Disorder-Associated MicroRNA

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00424E, Paper

Wei Li, Xiaobin Dou
Tic disorders (TDs) are prevalent neuropsychiatric conditions in children, yet early diagnosis remains challenging due to limited availability and high sequence homology of associated microRNA biomarkers. Existing detection methods often...
The content of this RSS Feed (c) The Royal Society of Chemistry

Accurate quantification of polyester in textiles through complete depolymerisation and HPLC

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00086J, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Mattia Cerri, Leena Pitkänen, Corné Schutgens, Michael Hummel
PET content in textiles is accurately quantified through depolymerisation to terephthalic acid followed by HPLC.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Sensitive detection of hesperidin in citrus peel using nitrogen doped mesoporous carbon/metal–organic framework composite-modified electrodes

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00309E, Paper

Wei Liu, Yanhua Sun, Zhiyong Zhu, Zi Li, Hongfang Li, Qiongyao Zeng
This study synthesized nitrogen-doped mesoporous carbon (NMC) using Zn-MOF-8 as a template, followed by the preparation of ZIF-08/NMC composites via π–π stacking interactions between ZIF-08 and NMC.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Rapid miniaturized electrochemical biosensor based on screen-printed technology for early-stage taurine detection through manganese molybdate nanosheets and bionicimprinted nanofilms

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02145F, Paper

Yudong Sun, Yuanyuan Jin, Yirui Liu, Lei Zhu, Cheng Zhang, Danfeng Yu, Jiasheng Xu, Mingkai Fu
This work presents a compact, portable bioelectrochemical sensor for sensitive, selective taurine detection. Its miniaturized design, high integration, and simple fabrication show promise for POC bioanalysis.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

One-pot iron determination in fortified flours using thymol-1,10-phenanthroline deep eutectic solvent and smartphone-based digital image photometry

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00299D, Paper

Samara Soares, Fabio Rodrigo Piovezani Rocha, Maria C. Hespanhol
Iron fortification of flours is an important public health strategy for reducing iron-deficiency anemia. Conventional procedures for iron determination in fortified flours generally involve acid digestion followed by determination using...
The content of this RSS Feed (c) The Royal Society of Chemistry

Chemometric Evaluation of the CUPRAC Assay for Propolis Quality Control: A Decision-Making Framework for Deciphering Antioxidant Paradoxes

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00251J, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

mehmet beykaya, Aslı Elif Tanuğur Samancı, TAYLAN SAMANCI, ELİF YORULMAZ ÖNDER
The antioxidant capacity of propolis is traditionally inferred from single compositional indicators such as total phenolic (TPC) or flavonoid content (TFC). However, these reductionist approaches often overlook complex inter-compound dynamics,...
The content of this RSS Feed (c) The Royal Society of Chemistry

Analysis of the effect of glycerol addition and heating temperature on the puff-by-puff release of volatile organic compounds from heated tobacco products

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00249H, Paper

Ting Fei, Junjie Li, Bingyu Wu, Jie Yu, Peicai Cui, Yunfei Sha, Qian Cheng, Min Chen, Yicheng Fang, Jing Yang
Large-volume thermal injection-gas chromatography-mass spectrometry was used to investigate how glycerol addition and heating temperature affect the puff-by-puff release of volatile organic compounds in aerosols from HTPs.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Catalytic hairpin assembly-assisted split-T7 promoter-regulated CRISPR/Cas12a system for the sensitive analysis of microRNAs associated with coronary heart disease

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00524A, Paper

Xiuju Zhang, Xiaowei Nie, Wenxiao Yu, Guanchao Du, Shaoming Liu, Yewen Song
MicroRNAs (miRNAs) have emerged as promising non-invasive biomarkers for coronary heart disease (CHD); however, their accurate quantification remains challenging due to their short length, low abundance, and high sequence homology.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Reverse micelle-assisted dispersive liquid–liquid microextraction for trace determination of methylene blue in aqueous samples

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01840D, Paper

TingTing Yu, ChuanYing Deng, Feng Hu, SiQi Yang, Rui Liang, Yi Liu, Nan Hu, YongMei Chen, XiaoLin Yu
By leveraging the hydrophilic cores and extensive contact surface areas, a reverse micelle-assisted dispersive liquid–liquid microextraction method achieves a detection limit of 0.18 ng mL⁻¹ for trace methylene blue analysis in aqueous samples.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Co–Cu bimetallic MOF-derived nanozyme-based colorimetric immunoassay with smartphone-based RGB readout for sensitive detection of alpha-fetoprotein

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00326E, Paper

Shengyi Fang, Sheng Ye, Dan Ouyang, Songyan Tie, Jianzhong Cao
This manuscript reports Co–Cu bimetallic MOF-derived nanozymes for colorimetric immunoassay of alpha-fetoprotein, employing smartphone-based RGB readout.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Pt@MOF-derived branch-leaf nanoarray: a mechanically flexible and high-performance biosensor for non-enzymatic electrochemical glucose detection

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00106H, Communication

Chongyu Liu, Li Ren, Weihua Zhuang, Xiaoxi Guo, Yongchao Yao, Jie Hu, Xuping Sun, Wenchuang (Walter) Hu
Developing mechanically flexible nanoarray electrodes with strong catalytic performance is necessary for non-enzymatic glucose biosensing. Herein, we present a self-supported Pt@MOF-derived branch-leaf nanoarray that functions as an efficient electrode for...
The content of this RSS Feed (c) The Royal Society of Chemistry

A coumarin-based colorimetric receptor coupled with a smartphone for the determination of fluoride concentration in drinking water

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00116E, Paper

Pingzhang Gao, Shi-Ya Peng, Xuefen Pi, Xiao-Lan Xie, Rong Tian, Naihao Lu
A simple smartphone-assisted colorimetric method was designed to determine fluoride concentrations in drinking water using a coumarin-based receptor.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Rapid identification of automotive window film brands via hyperspectral imaging with two-stage feature selection

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00230G, Paper

Jian-Jun Xu, Si-Yuan Shen, Lu-Lu Jiang, Yong He, Yi-Fan Jiang
This study presents a hyperspectral imaging-based method for identifying automotive window film brands.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A molecularly imprinted polymer-based electrochemical sensor for the detection of colistin in milk

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02101D, Paper

Ashish K. Singh, Supriya Sharma, Divya Verma, Deepali Tripathi, Sucharita Sen Maiti, Sandeep K. Sharma, Smriti Priya
MIP based electrochemical detection method for Colistin, a peptide-based antibiotic used against multidrug-resistant Gram-negative bacterial infections.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Development of a rapid and sensitive single-tube RAA-CRISPR/Cas12a assay for monkeypox virus detection

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00065G, Paper

Fan Yu, Duo Zhang, Chengcheng Peng, Huiling Qin, Fei Xu, Yuyuan Zhang, Zhicheng Pan, Pengpeng Xiao, Nan Li
A rapid, single-tube RAA-CRISPR/Cas12a assay provides sensitive and specific detection of Monkeypox virus (MPXV) with simplified procedures, ideal for point-of-care use.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Rational design of environment-sensitive fluorescent probes for butyrylcholinesterase and their application in biological imaging

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00158K, Paper

Lulian Shao, Feng Wang, Yu Wang, Jiahao Du, Wenting Cao, Qingwen Zhang, Fenqin Zhao, Junfeng Wang, Wenhui Fan, Yuemin Zhou, Bingxin Wang, Xiaobin Pang, Lin Yan
Rational design of environment-sensitive fluorescent probes for butyrylcholinesterase and their application in biological imaging.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Green analytical approach for multielemental determination in oily matrices combining extraction induced by emulsion breaking (EIEB) with ICP OES

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00110F, Paper

Maria Isadora Pereira Lima, Lucas Rafael de Moura Domingos, Naomi Akiba, Ivanise Gaubeur
Extraction induced by emulsion breaking: a low-impact, solvent-saving analytical method for oil sample preparation and multielement metal determination by emulsion techniques.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Development of an effective preconcentration strategy for the trace determination of nickel in thyme tea extracts by FeSbO4 nanoparticle-based dispersive solid phase extraction (DSPE)

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02102B, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Muhammed Ali Büyük, Hakan Serbest, Sezgin Bakırdere
FeSbO₄ nanoparticles were fabricated under hydrothermal synthesis conditions and utilized for the first time as sorbent materials for the preconcentration/separation of Ni ions.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A unified UPLC-MS/MS method for simultaneous quantification of intact anthocyanins and their metabolites in rat plasma

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00186F, Paper

Chunzi Zhang, Guzhalinuer Aierken, Xiaoyan Ma, Tuxia Pang, Juan Zhang, Mihriban Arken, Shuyong Yu, Qianghui Mian, Xiaoli Ma
This study developed and validated a rapid UPLC-MS/MS method for the simultaneous determination of anthocyanins and their metabolites in rat plasma.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Beyond identification: inferring physical activity from fingerprint lipids using machine learning

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02066B, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Daphne R. Patten, Raven L. Buckman Johnson, Trevor T. Forsman, Young Jin Lee
Beyond identifying a person, fingerprints contain chemical profiles that machine learning can use to reveal physical activity.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Construction of hollow fiber stirring bar based on dummy molecularly imprinted polymers for the selective determination of organophosphorus pesticides in food samples

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00280C, Paper

Jia Li, Binlian Jiang, Runan Chen, Yanjiao Qi, Dongdong Cheng, Jun Lin, Zegen Wang, Lina Lu, Shuhe Kang, Xiaojun Yang
The sol–gel method and layer-by-layer self-assembly were used to graft dummy template molecularly imprinted polymers onto CG161M microspheres, yielding DMIPs@CG161M as an adsorbent for the detection of organophosphorus pesticides in food.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Construction of a label-free COF/PTh/MB electrochemical immunosensor for the detection of CA-125

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00265J, Paper

Jiajia Wang, Chun Kan, Demeng Qian, Mengping Gao, Ayang Zhou, Baokang Jin
The combination of PTh with MB wasincreased the specific surface area and enhance the electron transfer ability, PTh/MB was combined with COF improved the material's stability. The resulting COF/PTh/MB achieved highly sensitive detection of CA-125.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

An orthogonal extraction workflow for high-depth plasma proteomics with metabolomic compatibility from low microliter-scale samples

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00195E, Paper

Chuanping Zhao, Huiting Hu, Xiaohong Qian, Wanjun Zhang, Weijie Qin
An orthogonal extraction workflow enables deep plasma proteome coverage and metabolomic compatibility from only 10 µL plasma, improving low-abundance protein detection while preserving robust metabolite profiling for integrated biomarker discovery.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Innovative method orange gradient index (IMOGI): a new tool for assessing analytical methods in terms of innovation and modernization

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00317F, Paper

Heba Shaaban
IMOGI (Innovative Method Orange Gradient Index) is a visual framework for evaluating innovation in analytical methods using ten objective criteria. A concentric orange-gradient chart generates a transparent score (0–100), enabling rapid benchmarking of methodological innovation and modernization.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Exploring potential quality markers of paeoniae radix alba by UPLC-Q-TOF/MS, HPLC, and cell experimentation

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01846C, Paper

Jingbo Yu, Junpeng Long, Fang Long, Yang Sun, Ziyang Zhou, Ying Zhang, Guangli Yan, Yue Han
The workflow of exploring potential quality markers of paeoniae radix alba by UPLC-Q-TOF/MS, HPLC, and cell experimentation.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Magnetic chitosan/ZIF-L/KCDs granular-leaf hybrid nanocomposite for magnetic dispersive µsolid phase extraction of mycotoxins from ziziphus jujuba, nuts and grains

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00202A, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Mahboube Shirani, Qamar Salamat, Mohammadjavad Jahanshahi, Soheila Sepahi, Mustafa Soylak
A novel magnetic chitosan/zeolitic imidazolate frameworks-L/kiwi peel-derived carbon dots (MCS/ZIF-L/KCDs) granular-leaf hybrid nanocomposite was used for MD-µSPE of aflatoxins prior to HPLC-FLD analysis.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Nitrogen doped carbon dots with antibacterial activity for dual mode fluorescence and smartphone-assisted colorimetric detection of epirubicin

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01270H, Paper

Sanhita Swain, Swagatika Dehury, Santosh Kumar Sahu, Indrani Mohanty, Swagatika Sahu, Ashis Kumar Jena
Nitrogen-doped carbon dots have been employed for dual mode fluorometric as well as smartphone assisted colorimetric detection of epirubicin and showed antibacterial activities.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Multifunctional CuS-CDs@MnO2 nanoparticles and liposome-assisted amplification for triple-mode detection of enrofloxacin

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00118A, Paper

Luna Guo, Huanhuan He, Ruoyan Li, Shiyi Dai, Jing Feng, Guangshuo Zhang, Zixian Li, Weiling Song, Hong Zhou
Multifunctional mesoporous silica nanosphere driven self-powered photoelectrochemical/electrochemical dual-mode for sensitive TNF-α detection.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Rapid identification of chemical constituents of Liaogu Formula based on UHPLC-Q-TOF-MSE combined with UNIFI platform

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D5AY02031J, Paper

Xin Li, Caiying Liu, Liqi Ng, Qiujia Yu, Yingtao Bai, Yu Zhou
Chemical components of Liaogu Formula (LGF) were analysed using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UHPLC-Q-TOF-MSE) combined with the UNIFI platform. The analysis was conducted using an InfinityLab Poroshell...
The content of this RSS Feed (c) The Royal Society of Chemistry

Multidimensional information detection of liver cancer cell exosomes treated with Shikonin

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00133E, Paper

Jian Qiao, Jingran Zhang, Chunli Qin, Changji Mu, Tuoyu Ju, Yuanting Yang, Yang Liu, Liguo Tian, Yanquan Geng
Exosomes were recently recognized with growing attention as mediators of intercellular communication and as potential biomarkers associated with cancer.However, the multidimensional characterization of exosomes secreted by cancer cells after drug...
The content of this RSS Feed (c) The Royal Society of Chemistry

MIL-53-PVDF mixed-matrix membrane for dispersive membrane solid-phase extraction of phenoxy carboxylic acid herbicides in environmental water sample followed by HPLC determination

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00059B, Paper

Ronghui Xiao, Mingshan Jiang, Jiping Ma
The MIL-53-PVDF mixed-matrix membrane was prepared for the enrichment of four phenoxycarboxylic acid herbicides (2,4-D, MCPA, 2,4-DP, 2,4-DB) in environmental water samples, and the enriched analytes were determined by high...
The content of this RSS Feed (c) The Royal Society of Chemistry

UV-Vis determination of acetate in ethanol electrolysis for renewable hydrogen production

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00079G, Paper

Zuyao Li, Jing Li, Chaoyang Jiang, Guiyun Liu, Zihan Fang, Zhenchen Tang, Huanhao Chen, Hai Cao, Feng Zeng
Electrochemical oxidation of ethanol can simultaneously produce hydrogen and value-added acetate, but accurate quantification of acetate in ethanol-rich, high-ionic-strength alkaline electrolytes remains challenging. Here we report a cost-effective, sensitive UV-Vis...
The content of this RSS Feed (c) The Royal Society of Chemistry

Capillary electrophoresis-contactless conductivity detection for routine screening of PDE-5 inhibitor adulterants in sexual-enhancement supplements

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00247A, Paper

Thi Anh Huong Nguyen, Khanh Hung Nguyen, Quoc Anh Hoang, Cao Son Tran, Thi Trang Vu, Viet Chien Dinh, Thi Phuong Quynh Le, Thi Thuy Duong, Anh Phuong Vu, Thi Ngoc Mai Pham, Bach Pham, Thi Hong Hao Le
A CE-C⁴D method was developed as a low-cost alternative to HPLC for screening PDE-5 inhibitor analogs in supplements. Undeclared sildenafil and homosildenafil were found in male sexual-enhancement products at up to 58.4 and 16.1 mg g⁻¹, respectively.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Analysis of diazepam residues in aquatic products based on magnetic molecularly imprinted polymers

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00207B, Paper

Zhicen Liu, Boxuan Yao, Hangfei Gao, Long Cheng, Shuyan Bai, Chenhui Li, Honghua Zhao, Yueping Sun, Aimin Wang, Dongli Qin, Li Huang, Lei Gao
This study successfully prepared an efficient magnetic molecularly imprinted polymer (MMIP) nanocomposite using carbon nanotubes as the carrier.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A green SPE sorbent based on butterfly pea anthocyanin extract immobilized on Amberlite XAD-1600N for Pb(II) determination by FAAS

Anal. Methods, 2026, 18,2734-2747
DOI: 10.1039/D5AY02013A, Paper

Sureerat Sanguthai, Bualan Khumpaitool, Khwanthipha Pandecha
Lead contamination in seafood poses a significant risk to human health, necessitating reliable and environmentally sustainable analytical methods for routine Pb(II) monitoring.
The content of this RSS Feed (c) The Royal Society of Chemistry

Plasma NIR spectral pattern recognition applied for rapid screening of overt and occult HBV infections

Anal. Methods, 2026, 18,2704-2714
DOI: 10.1039/D5AY02111A, Paper

Xiaoting Huang, Linbin Huang, Jiayi Li, Min Chen, Meijun Chen, Huanjie Zhou, Baoren He, Chao Ou, Tao Pan
Plasma near-infrared (NIR) spectral pattern recognition can accurately perform two-classification and three-classification discrimination of hepatitis B surface antigen positivity (HBsAg⁺), occult HBV infection (OBI), and healthy individuals.
The content of this RSS Feed (c) The Royal Society of Chemistry

Fabrication of a highly sensitive and stable fluorinated xerogel/cellulose acetate film optical sensor for application in dissolved oxygen detection

Anal. Methods, 2026, 18,2632-2639
DOI: 10.1039/D6AY00197A, Paper

Gang Xu, Baojun Sun, Xiaowei Zhang, Jie Gao, Xiaomei Li, Limin Bai, Ying Zhang, Shihan Li, Yang Wang
A highly sensitive and stable optical fiber sensor based on fluorinated xerogel/cellulose acetate incorporating ruthenium compounds was fabricated for dissolved oxygen detection.
The content of this RSS Feed (c) The Royal Society of Chemistry

TransCNN: a hybrid deep learning model for detecting honey adulteration by LED-induced fluorescence spectroscopy

Anal. Methods, 2026, 18,2692-2703
DOI: 10.1039/D5AY02014J, Paper

Zhijian Liu, Lanjun Sun, Xiongfei Meng, Le Li, Lin Wang
A hybrid TransCNN model integrates convolutional feature extraction with transformer-based global attention, enabling rapid and accurate detection of honey adulteration by LED-induced fluorescence.
The content of this RSS Feed (c) The Royal Society of Chemistry

Spectrophotometric determination of the degree of acetylation of cellulose acetate via ferric hydroxamate complexation: method development and validation

Anal. Methods, 2026, 18,2765-2773
DOI: 10.1039/D5AY02011E, Paper

Hyok-Sim Han, Hyon-Tae Pak, Kyong-Sik Ju, Kum-Hak Pak, Jong Nam Kim
A rapid, accurate, and cost-effective spectrophotometric method has been developed for determining the degree of acetylation (DA) of cellulose acetate (CA).
The content of this RSS Feed (c) The Royal Society of Chemistry

Eu-functionalized hydrogen-bonded organic frameworks as a ratiometric fluorescent sensor for highly sensitive detection of zearalenone

Anal. Methods, 2026, 18,2622-2631
DOI: 10.1039/D6AY00268D, Paper

Hou-Qun Yuan, Cong-Cong Xu, Qiong-Yue Cui, Guan-Qun Li, Yuan-Lei Zhang, Yi-Fan Xia, Lan Yang, Hua Yu, Yan-Xia Li, Guang-Ming Bao
A europium-functionalized HOF (Eu@HOF-TMA) was fabricated and developed as a fluorescent sensor for ratiometric detection of zearalenone (ZEA) with high selectivity and sensitivity. An instrument-free visual detection method for ZEA was constructed.
The content of this RSS Feed (c) The Royal Society of Chemistry

Single-enzyme-driven cascade amplification integrating target recycling and a DNA walker for highly sensitive nucleic acid detection

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00143B, Paper

Yizhuo Zhao, Mengying Gao, Jiabei Xing, Ya Li, Xianchao Du, Huanhuan Xing, Xueguo Liu, Dongfang Qiu, Xiaojing Xing
A single-enzyme cascade amplification strategy was developed using Exonuclease III to drive both target recycling and a DNA walker. The method avoids multiple enzymes and sequence-specific recognition, enabling sensitive detection of nucleic acid.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A room temperature synthesized SERS substrate of AgNPs/PVA hydrogel for highly sensitive detection of enrofloxacin using a portable Raman system

Anal. Methods, 2026, 18,2659-2669
DOI: 10.1039/D5AY02061A, Paper

Qingli Xie, Xueyang Wang, Boxue Chang, Yinlan Ruan
A flexible AgNPs/PVA hydrogel substrate provides uniform and stable SERS enhancement, enabling ultrasensitive detection of organic dyes and antibiotics.
The content of this RSS Feed (c) The Royal Society of Chemistry

Optimised untargeted metabolomics workflow for human urinary extracellular vesicles

Anal. Methods, 2026, 18,2748-2764
DOI: 10.1039/D6AY00002A, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Cahyani Gita Ambarsari, Sandra Martinez-Jarquin, Jasper J. R. Koh, Grace Needham, Kenton P. Arkill, Victoria James, Maarten W. Taal, Jon Jin Kim, Dong H. Kim, Anna M. Piccinini
Extracellular vesicles (EVs) have been a key focus in biomarker discovery, with urinary EVs (uEVs), primarily derived from cells of the urogenital tract, providing valuable insights into kidney and urinary tract health and disease.
The content of this RSS Feed (c) The Royal Society of Chemistry

Deep learning-empowered SERS: deciphering the multidimensional information code of complex biological samples

Anal. Methods, 2026, 18,2575-2614
DOI: 10.1039/D6AY00224B, Critical Review

Biqing Chen, Xiaohong Qiu
Deep learning-empowered SERS decodes multidimensional biochemical information from complex biological samples. Integrating spectral processing and AI analysis, this review addresses strategies, challenges and prospects for clinical SERS-AI systems.
The content of this RSS Feed (c) The Royal Society of Chemistry

Assessing the feasibility of Fenton's reagent for microplastic extraction from mussels and its application to coastal pollution monitoring in Zhejiang, China

Anal. Methods, 2026, 18,2715-2724
DOI: 10.1039/D5AY01760B, Paper

Hui Huang, Zhen Wu, Kai Song
Fenton's reagent enables efficient (≥88%), non-destructive microplastic extraction from mussels. Application along Zhejiang coast revealed 1.8–9.0 items per g tissue, predominantly CPE (40.7%) and PVC (32.2%). This protocol supports standardized mussel-based biomonitoring of microplastic pollution.
The content of this RSS Feed (c) The Royal Society of Chemistry

Optimisation of through-vial Raman analysis of turbid samples by modelling the Raman intensity-depth decay response

Anal. Methods, 2026, 18,2682-2691
DOI: 10.1039/D6AY00130K, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Vasundhara Tyagi, Alexander G. Shard, Ryan T. Coones, Dimitrios Tsikritsis, Enya Murray, Elizabeth Legge, Caterina Minelli, Natalie A. Belsey
Predicting Raman signal decay in particulate samples for optimised quantitative measurements.
The content of this RSS Feed (c) The Royal Society of Chemistry

SERS-assisted multivariate data analysis of the spectinomycin-Cu(II) complex released in blood serum from a stimuli-responsive drug carrier: in vitro kinetics modeling and pharmacodynamic analysis

Anal. Methods, 2026, 18,2640-2658
DOI: 10.1039/D5AY02042E, Paper

Rayan Y. Mushtaq, Abdul Naman, Nasir Mehmood, Mohammed Alissa, Ghadah S. Abusalim, Musaad M. Althobaiti, Minseok Kim
Controlled drug delivery from stimuli-responsive drug carriers offers effective therapeutic potential; however, the precise assessment of the release kinetics and pharmacodynamic (PD) behavior remains challenging.
The content of this RSS Feed (c) The Royal Society of Chemistry

Ecologically benign S-doped g-C3N4 nano-architectonics for rapid and selective fluorescent detection of the jaundice biomarker bilirubin

Anal. Methods, 2026, 18,2786-2798
DOI: 10.1039/D5AY02080H, Paper

Govindha Pandi Rajaram, Murugan Veerapandian, Shanmugam Senthil Kumar
Label-free biomolecular sensor probe without complex pre-treatment offers potential for advanced point-of-care clinical diagnosis.
The content of this RSS Feed (c) The Royal Society of Chemistry

Waste to resource approach: CoFe2O4-modified spent tea waste for magnetic solid-phase extraction of toxic metal ions from water samples

Anal. Methods, 2026, 18,2799-2811
DOI: 10.1039/D6AY00152A, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Serdal Seker, Duygu Ozdes, Celal Duran
Tea waste-derived biochar magnetically modified with CoFe₂O₄ enables ultrafast and efficient solid-phase extraction of Cd(II), Pb(II), and Ni(II) ions from environmental waters, offering a low-cost and sustainable adsorbent.
The content of this RSS Feed (c) The Royal Society of Chemistry

Simultaneous electrochemical detection of dopamine and paracetamol using a carbon paste electrode modified with neodymium oxide and prepared via hydrothermal treatment

Anal. Methods, 2026, 18,2725-2733
DOI: 10.1039/D5AY02082D, Paper

Gloria A. Cosco-Salgero, Eduardo Salazar, Johisner Penagos-Llanos, Rodrigo Segura, Edgar Nagles, John J. Hurtado
In this report, hydrothermal treatment of a carbon-based electrode modified with neodymium oxide markedly enhanced the electroactive and conductive properties of the electrode.
The content of this RSS Feed (c) The Royal Society of Chemistry

Bisbenzimidazoles with a benzenediyl spacer: an efficient fluorophore for dihydrogen phosphate sensing and targeted living cell imaging

Anal. Methods, 2026, 18,2670-2681
DOI: 10.1039/D5AY02020D, Paper

Rakesh Debnath, Subhajit Saha, Subhankar Kundu, Abhranil De, Sourav Mondal, Muddukrishnaiah Kotakonda, Nilanjan Dey, Bhaskar Biswas
A bisbenzimidazole-based fluorescent probe (PBZ) has been developed for the selective detection of dihydrogen phosphate (H₂PO₄⁻) ions.
The content of this RSS Feed (c) The Royal Society of Chemistry

Precision traceability of trace psychoactive substances under water quality parameter perturbations in a complex matrix situation

Anal. Methods, 2026, 18,2774-2785
DOI: 10.1039/D5AY01865J, Paper

Zheng-jie Han, Meng-yue Fang, Yan Zhang, Dao-ling Wang, Jie Hou, Hao-chen Yan, Kai-xuan Luo, Xian-guo Li, Da-hai Zhang
Wastewater-based drug testing accuracy is affected by substance degradation during transport. Multivariate regression models using COD, TN, and NH₃–N reduce estimation deviation from 67.37–98.15% to 12.98–29.85% via direct measurement, significantly improving abuse assessment.
The content of this RSS Feed (c) The Royal Society of Chemistry

A novel magnetic and fluorescent nanocomposite probe for detection of matrix metalloproteinase-2 in cells

Anal. Methods, 2026, 18,2615-2621
DOI: 10.1039/D5AY01892G, Paper

Yidan Liu, Chunyang Kang, Xianwei Zuo, Lei Zhao, Yan Wang, Chunyan Liu, Bo Wang
This study presents a novel magnetic-fluorescent nanocomposite probe for MMP-2 detection, which was innovatively developed by self-assembly of a specifically designed MMP-2 substrate peptide and cobalt-nitrilotriacetate modified magnetic beads.
The content of this RSS Feed (c) The Royal Society of Chemistry

DNA-mediated synergistic proximity ligation for ultrasensitive electrochemiluminescence detection of microcystin-LR

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00161K, Paper

Hongfang Gao, Rong Xiao, Taotao Ma, Xiaolin Yang, Shangxian Ma
An innovative ECL aptasensor based on a DNA-mediated synergistic proximity cooperative complex comprising three ferrocene groups was developed for MC-LR detection.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Breaking the Bottlenecks in Nanozyme-Antibody Conjugates Preparation: Challenges, Optimization Strategies, and Applications of in Vitro Diagnostics

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00209A, Critical Review

Kehui Yu, Mengjun Wang, Haoan Wu, Ming Ma, Yu Zhang
Enzyme-labeled antibodies serve as the fundamental pillars of immunoassay-based biosensing, providing the necessary molecular recognition and signal amplification for the detection of trace analytes in complex matrices. As robust mimics...
The content of this RSS Feed (c) The Royal Society of Chemistry

A TiO2/Ti3C2 MXene photoelectrochemical chip with multiphysics field engineering for quantification of wastewater DNA

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00099A, Paper

Qitong Zeng, Xiaojie Ma, Tianci Liu, Zedong Liang, Shengxin Liu, Shuohua Zhang, Changbiao Chi
This study develops a TiO₂/Ti₃C₂ MXene heterojunction-based photoelectrochemical microfluidic chip enhanced by acoustic and magnetic multiphysics fields for ultrasensitive detection of wastewater DNA.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Rapid point-of-care detection of vitamin D deficiency using disposable electrochemical immunosensors based on a La2O3-WO3@CNT Bi-metal oxide nanocomposite

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00076B, Paper

Omar Ramadan, Mostafa Ahmed, Pradeep Kumar Brahmana, Daohong Zhang, Rabeay Y. A. Hassan
The nanostructured sensing system is designed and fully optimized with vitamin D-specific biorecognition probes, then integrated onto a disposable substrate to fabricate a portable device for vitamin D level testing.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Quantification of microplastics in soil and sediments using dry ice assisted fractionation with an Agilent 8700 laser direct infrared chemical imaging system

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01753J, Paper

Subharthe Samandra, Jaye Marchiandi, Wesam S. Alwan, Amanda V. Ellis, Bradley O. Clarke
This study reports a novel protocol for the extraction and quantification of 14 different polymers in soil and sediments.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A Green Analytical Method for Identification and Quantification of Carcinogenic Impurity N-Nitroso-Desformyl Riociguat in Riociguat Formulations by UHPLC-MS/MS

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00279J, Paper

Veeraswami Boddu, Manoj Kumar Mandapaka
Riociguat, a vasodilatory drug for treating pulmonary hypertension (PH), might generate a Nitrosamine drug substance-related impurity (NDSRI), reported as N-Nitroso-Desformyl Riociguat (NNDFR); a similar class of nitrosamine impurities, which are...
The content of this RSS Feed (c) The Royal Society of Chemistry

Construction and application of a coumarin-based fluorescent probe for hypochlorous acid

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02143J, Paper

Yue Li, Yan Dong, Yingqian Xu, Junyi Hu, Jiaoyue Zhang, Ying Chen, Dengbin Wu
XDS acts as a fluorescent probe for HClO recognition. It has been successfully applied to the semi-quantitative detection of HClO in real water samples, as well as bioimaging of HClO in HepG2 cells and zebrafish.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Isothermal amplification and CRISPR/Cas one-pot detection systems: strategies and prospects

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01976A, Tutorial Review

Baoying Ma, Xiaodong Wang, Xiangjing Cai, Mianyun He, Shiying Lai, Pengfei Zhang, Junfa Xu
Schematic of one-pot isothermal amplification–CRISPR/Cas detection. Targets are amplified (RPA 37–42 °C or LAMP 60–65 °C), followed by Cas-mediated trans-cleavage to generate signals via reporter cleavage, enabling flexible readouts.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A novel NIR fluorescent probe based on p-mercaptophenylboronic acid-functionalized copper nanoclusters for capmatinib quantification

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01825K, Paper

Ali O. Alqarni, Rayed Ali A. Alqahtani, Ashraf M. Mahmoud, Yahya S. Alqahtani, Bassam S. M. Al Kazman, Fatmah M. Alshareef, Mohamed N. Goda, Laila S. Alqarni, Ramadan Ali, Al-Montaser Bellah H. Ali, Mohamed M. El-Wekil
A red-emitting p-MPBA@CuNC “turn-off” fluorescent probe was developed for highly sensitive and selective capmatinib detection.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Oxygen concentration measurement in 3D cell culture using multifocal optical projection microscopy

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01921D, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Birhanu Belay, Mart Kroon, Kaisla Walls, Minna Kellomäki, Jari Hyttinen
Fluorescent intensity-based oxygen concentration measurement in large-volume 3D cell cultures using microsensor beads and multifocal optical projection microscopy.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Validation of an HS-SPME-GC-MS method for the analysis of multiple fumigants in low moisture agricultural commodities

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01636C, Paper

Wiley A. Hall, Kylie Stiglmeier, Thomas Jones
A single method for the analysis of multiple fuimgant residues in low moisture commodity is presented.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Dual-mode flexible surface-enhanced Raman spectroscopy-based smart tape for thiram and thiabendazole detection

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01989C, Paper

Nan Wang, Jing Wang, Hongfei Liu, Ruxi Huang, Jun Lu, Duo Lin
A flexible surface-enhanced Raman spectroscopy (SERS)-based tape was developed for sensitive detection of commonly used pesticides.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Liquid chromatography-mass spectrometry study of the composition and structure of alkyl salicylate as a detergent additive in lubricants

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01988E, Communication

Guiying Kang, Wei Cheng, Jin Li, Dahua Zhang, Yufeng Liu
The high-temperature detergency of alkyl salicylates (ASs) as lubricant detergents is influenced by their composition and molecular structure.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Construction of an electrochemiluminescence immunosensor based on a pre-concentration enhanced mechanism for ultrasensitive detection of CEA

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00041J, Paper

Yumeng Jiang, Haoyi Ren, Chenglin Hong, Mingzhe Jiang
A sensitive ECL immunosensor for CEA using CDZS-1 as Ru(bpy)₃²⁺ carrier and CuO NPs as co‑reaction accelerators achieved a very low detection limit and wide linear range, showing potential for colorectal cancer diagnosis.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

The art of time travel: exploring radiocarbon dating in cultural heritage

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY90027E, AMC Technical Brief

Analytical Methods Committee, AMCTB No. 122
Radiocarbon dating helps establish timelines in cultural heritage by analysing carbon in materials such as wood, textiles, paper, parchment and lacquer, aiding authentication and historical research.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A low-cost, high-throughput DNA quantification system using light pipe arrays for parallel fluorescence measurement

Anal. Methods, 2026, Accepted Manuscript
DOI: 10.1039/D6AY00434B, Paper

Jiandong Zhu, Haojie Sun, Bo Yang, Zhenqing Li, Yoshinori Yamaguchi
Accurate DNA concentration measurement is essential for molecular biology, enabling precise quantification and optimization of various experimental protocols. While UV absorbance at 260 nm is common, fluorometric methods using fluorescent...
The content of this RSS Feed (c) The Royal Society of Chemistry

High-throughput colorimetric LAMP detection of Mycoplasma gallisepticum with intelligent algorithm-assisted analysis

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00198J, Paper

Wuyang Jing, Qianyi Cai, Yucen Liang, Renhang Xiao, Qijie Lin, Xiaozhen Xu, Ruidong Liu, Chenggang Xu, Hongchao Gou, Haiyan Shen, Ming Liao, Jianmin Zhang
A colorimetric LAMP assay using cresol red enables visual detection of Mycoplasma gallisepticum, while an intelligent smartphone application (Lab Color Analyzer) automates analysis of up to 64 samples, achieving 98% concordance with qPCR.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Identifying small molecule impurities in electrospun poly(vinyl alcohol) nanofibres using ultra-selective NMR

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY02043C, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Robert Evans, W. Joseph A. Homer, Alice Millbank, Bridget Tang, Hana Ali, Maxim Lisnenko, Eva K. Kostakova, Jan Valtera, Vera Jencova, Ralph W. Adams, Paul D. Topham, Eirini Theodosiou
Ultra-selective GEMSTONE NMR successfully identifies small molecule impurities present in electrospun polymer mats.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Rapid ultrasensitive detection of levamisole and mebendazole residues in meat products based on an intelligent nanozyme-imprinted fluorescence microfluidic sensor

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00111D, Paper

Qiuyu Wu, Zhihui Liao, Yujin Ouyang, Ao Ma, Zhaoxia Yang, Zhaohui Zhang
An intelligent dual-nanozyme imprinted dual-ratiometric fluorescence paper-based microfluidic sensor, combined with smartphone readout and MATLAB-based analysis for rapid, simultaneous, ultrasensitive determination of LMS and MBZ in meat products.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Self-powered PEC/EC dual-mode sensing platform for sensitive TNF-α detection based on multifunctional mesoporous silica nanospheres

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00126B, Paper

Jing Feng, Huanhuan He, Luna Guo, Weiling Song, Hong Zhou
A multifunctional mesoporous silica nanosphere driven self-powered photoelectrochemical/electrochemical dual-mode platform for sensitive TNF-α detection.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Nature's touch in the laboratory: eco-friendly and green dual-mode UV-vis and smartphone RGB analysis of procaine in pharmaceuticals using a non-heated aqueous matcha green tea extract

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00184J, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Batuhan Yardımcı
A novel green dual-mode method was developed for procaine determination using a non-heated matcha green tea extract as a natural diazo-coupling reagent, enabling simple, eco-friendly spectrophotometric and smartphone-assisted colorimetric analyses.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Avoiding and reducing microplastic false positives from dry glove contact

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D5AY01801C, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Madeline E. Clough, Eduardo Ochoa Rivera, Abbygail M. Ayala, Rebecca L. Parham, Joseph Pennacchio, Henry E. Thurber, Andrew P. Ault, Ambuj Tewari, Anne J. McNeil
Dry contact between gloves and lab equipment can lead to microplastic false positives. Here, we provide guidance on reducing this contaminant and develop methods that accurately quantify environmental microplastics.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

β-Cyclodextrin based host–guest complexation of 3-(2-pyridyl) acrylic acid for stability enhancement and aqueous-phase ascorbic acid detection

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00192K, Paper

Kangkan Mallick, Doli Roy, Priyanka Roy, Taniya Dey, Shilpi Ghosh, Subhankar Choudhury, Nitish Roy, Mahendra Nath Roy
Encapsulation of 3-(2-pyridyl)acrylic acid within β-cyclodextrin enhances stability and enables sensitive, selective fluorescence detection of ascorbic acid in aqueous media and real samples.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Ru–Au nanocluster-functionalized carbon fiber microelectrode enables ultrasensitive multiplexed monitoring of acetaminophen and its genotoxic interplay with DNA

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00071A, Paper

Tao Fang, Min Shi, Uchkun Ishimov, Awais Ihsan, Ruiting Tian, Jun Li, Xianju Huang, Han Cheng
A Ru–Au nanocluster-functionalized carbon fiber microelectrode was developed for sensitive simultaneous electrochemical detection of acetaminophen (ACOP) and its impurities. The platform also enables investigation of ACOP–DNA interactions.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

Correction: Multifunctional one-droplet microfluidic chemosensing of ractopamine in real samples: a user-oriented flexible nano-architecture for on-site food and pharmaceutical analysis using optical sensors

Anal. Methods, 2026, 18,2563-2563
DOI: 10.1039/D6AY90034H, Correction

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Hossein Navay Baghban, Mohammad Hasanzadeh
The content of this RSS Feed (c) The Royal Society of Chemistry

Rapid and sensitive fluorescence detection of clozapine via g-C3N4 nanosheet-based quenching

Anal. Methods, 2026, 18,2538-2547
DOI: 10.1039/D5AY02159F, Paper

Ya Zou, Zhouhong Tan, Jiaqian Li, Anzhong Peng, Jieli He
A highly sensitive g-C₃N₄ nanosheet-based fluorescent sensor for clozapine detection via charge-transfer quenching, enabling rapid therapeutic drug monitoring with a wide linear range of 0.02–500 µM and a detection limit of 3 nM.
The content of this RSS Feed (c) The Royal Society of Chemistry

Preparation of anti-idiotypic antibodies and their applications in mycotoxin detection: a review

Anal. Methods, 2026, 18,2380-2394
DOI: 10.1039/D5AY01936B, Critical Review

Zhuohui Pan, Jiacai Hu, Ziting Ou, Xiaodan Lv, Bo Hu, Junjun Huang, Zhili Xiao
Anti-idiotypic antibodies can simulate the functions of antigens and serve as replacements for antigens or toxin standards in mycotoxin detection immunoassays. These assays are referred to as “green immunoassays”.
The content of this RSS Feed (c) The Royal Society of Chemistry

CdSe/ZnS quantum dot-labeled antibody fluorescent immunoassay strip for swine acute diarrhea syndrome coronavirus S1 protein detection

Anal. Methods, 2026, 18,2511-2523
DOI: 10.1039/D5AY01694K, Paper

Bingxue Zhang, Gaiping Zhang, Jingming Zhou, Haili Wang, Hongliang Liu, Yumei Chen, Yanhua Qi, Xifang Zhu, Enping Liu, Sixuan Wu, Aiping Wang
Swine acute diarrhea syndrome coronavirus (SADS-CoV), a porcine enteropathogenic coronavirus, causes acute diarrhea, vomiting, and death in newborn piglets, leading to economic losses in the pig industry.
The content of this RSS Feed (c) The Royal Society of Chemistry

The analysis of 80 simple saccharides by ion chromatography mass spectrometry

Anal. Methods, 2026, 18,2491-2499
DOI: 10.1039/D5AY01489A, Paper

Open Access

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.

Hans S. A. Yates, James F. Carter, Jiali Zhang, Mary T. Fletcher, Viviene S. Santiago, Natasha L. Hungerford
The analysis of rare or atypical sugars is not encompassed by standard sugar methodology. With greater selectivity, this new method covers 80 sugars for their analysis.
The content of this RSS Feed (c) The Royal Society of Chemistry

Screening and characterization of anti-Listeria monocytogenes inlG-protein nanobodies and development of an ELISA method

Anal. Methods, 2026, 18,2480-2490
DOI: 10.1039/D6AY00135A, Paper

Ruoran Shi, Jingyao Liu, Honglin Ren, Yansong Li, Jiaqi Hou, Shaohui Hu, Yuzhu Wang, Chengwei Li, Bo Zhang, Ziqi Xing, Yuyang Ding, Fang Yang, Shiying Lu, Qiang Lu, Zengshan Liu, Xiaoxu Wang, Pan Hu
A high-specificity nanobody-based sandwich ELISA targeting the surface protein InlG was developed. This assay enables rapid, sensitive differentiation of pathogenic Listeria monocytogenes from non-pathogenic strains in complex food matrices.
The content of this RSS Feed (c) The Royal Society of Chemistry

Transwell-based analysis uncovers stimulus-specific chemotactic migration in Dictyostelium discoideum

Anal. Methods, 2026, Advance Article
DOI: 10.1039/D6AY00051G, Communication

Miranda Bravo, Antonia Ramos-Guzmán, Diego Rojas, Ian Pérez, Andrés E. Marcoleta, Francisco P. Chávez
We report an adapted Transwell® assay for robust and quantitative analysis of chemotactic migration in Dictyostelium discoideum.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry

A tip-on-tip micro-solid-phase extraction HILIC-LC-MS/MS platform for the determination of urinary methylated nucleosides

Anal. Methods, 2026, 18,2415-2428
DOI: 10.1039/D6AY00083E, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

C. Mena-Iglesias, A. Ballester-Caudet, D. García-Gómez, M. Bustamante-Rangel, E. Rodríguez-Gonzalo, J. L. Pérez Pavón
Development and validation of a sensitive HILIC-LC-MS/M method for the quantification of methylated nucleosides in urine. Sensitive and selective microscale solid-phase extraction using a tip-on-tip format was proposed as sample preparation strategy.
The content of this RSS Feed (c) The Royal Society of Chemistry

A fluorogenic and ultrabright probe for imaging of lipid droplets in live and fixed cells

Anal. Methods, 2026, 18,2548-2553
DOI: 10.1039/D6AY00188B, Paper

Guorui Zhao, Guanchang Ji, Dongdong Song, Yarong Ma, Guanzheng Tian, Guizhen Zhang
A new AIE probe TPE-Q targets lipid droplets with high specificity. The fluorescence turn-on is driven by a synergy of viscosity restriction and AIE, offering a robust tool for cell imaging.
The content of this RSS Feed (c) The Royal Society of Chemistry

Light-induced self-assembly of AgNPs for dual-range colorimetric detection of ethanol

Anal. Methods, 2026, 18,2500-2510
DOI: 10.1039/D6AY00015K, Paper

Jiahang Yu, Yimeng Huang, Ruihao Huang, Ke Zhao, Zongyou Ben, Xiao Sun
Rapid and accurate ethanol determination is crucial for quality control in the alcoholic beverage industry.
The content of this RSS Feed (c) The Royal Society of Chemistry

AFM-derived cytoskeletal mechanics reveal ethanol-induced injury and vitamin C-mediated repair in SH-SY5Y cells

Anal. Methods, 2026, 18,2407-2414
DOI: 10.1039/D5AY02053K, Paper

Xingyue Wang, Baohua Jia, Zuobin Wang, Kaige Qu
Oxidative stress and cytoskeleton remodeling are well established, but methods to assess cytoskeletal organization and mechanics remain invasive, hard to quantify dynamically, and dependent on fluorescence or electron microscopy.
The content of this RSS Feed (c) The Royal Society of Chemistry

Determination of vidarabine using fluorescein sodium probe-based resonance Rayleigh scattering

Anal. Methods, 2026, 18,2554-2562
DOI: 10.1039/D6AY00028B, Paper

Xinyu Yang, Meng Li, Na Yin, Nian Ding, Hong Yu, Jinyi Mao, Zhixun Yu, Qianqian Su
A resonance light scattering method for vidarabine determination was developed using fluorescein sodium probe, wherein the signal increased with vidarabine concentration (linear range: 0.5–5 mg·L⁻¹).
The content of this RSS Feed (c) The Royal Society of Chemistry

An “off-on” fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

Anal. Methods, 2026, 18,2455-2463
DOI: 10.1039/D6AY00068A, Paper

Zhuozhuo Ren, Qiongdan Zhang, Yuxing Wang, Zhuoer Chen, Ziyi Zhou, Da Qian
Schematic illustration of an “off-on” fluorescent nanosensor (OOFN) for sensitive and selective detection of APE1.
The content of this RSS Feed (c) The Royal Society of Chemistry

Synthesis of a hydrophilic covalent–organic framework@silica composite via an oxidation reaction for mixed-mode liquid chromatographic separation

Anal. Methods, 2026, 18,2472-2479
DOI: 10.1039/D6AY00067C, Paper

Xingyun Zhao, Jiangyan Jin, Peng Wang, Yonghao Chai, Kairui Zhu, Liyun Zhang, Ruifang Wang, Ke Li, Rongfang Wu
Oxidized imine-linked TAPT–TA-COF@SiO₂ was synthesized from 2,4,6-tris(4-aminophenyl-1,3,5-thiazine) (TAPT) and 1,4-benzenedicarboxaldehyde (TA) via one-pot cascade polymerization on silica spheres and used as a mixed-mode stationary phase.
The content of this RSS Feed (c) The Royal Society of Chemistry

An on-tissue chemical derivatization method for MALDI-MSI analysis of anthraquinones in mouse tissues

Anal. Methods, 2026, 18,2464-2471
DOI: 10.1039/D5AY01880C, Paper

Yong-An Yan, Si-Qi Han, Pian Jin, Xiao-Bo Zhao, Yan-Ping Shi, Wei Ha
A highly sensitive on-tissue chemical derivatization method was established for MALDI-TOF-MSI analysis of exogenous anthraquinones in mouse tissues.
The content of this RSS Feed (c) The Royal Society of Chemistry

Whole-cell SERS fingerprints for rapid toxicant attribution in water

Anal. Methods, 2026, 18,2445-2454
DOI: 10.1039/D6AY00054A, Paper

Ziyang Weng, Ying Zhou, Nan Ma, Meikun Fan
Whole-cell SERS fingerprinting enables rapid, label-free toxicant discrimination across heavy metal and organic pollutants using Gram-negative and Gram-positive bacterial sensors.
The content of this RSS Feed (c) The Royal Society of Chemistry

Cas13a/crRNA trans-cleavage triggered primer exchange reaction based self-priming chain extension for sensitive and label-free infantile pneumonia related microRNA analysis

Anal. Methods, 2026, 18,2524-2531
DOI: 10.1039/D6AY00090H, Paper

Jia Yang, Qiuling Huang
Accurate and sensitive detection of microRNAs (miRNAs) is crucial for both pathophysiological studies and clinical diagnostics.
The content of this RSS Feed (c) The Royal Society of Chemistry

Construction of a near-infrared fluorescent probe for cysteine and its application in oxidative stress research

Anal. Methods, 2026, 18,2532-2537
DOI: 10.1039/D6AY00075D, Paper

Lixia Zhang, Xingxing Zhao, Mei Yang, Siyu Yan, Xiaoxing Li, Jian Su, Hongmei Liu
The performance and biological applications of probe DC-Cys.
The content of this RSS Feed (c) The Royal Society of Chemistry

PVA/AgNP hydrogel SERS substrate combined with machine learning for highly sensitive detection of organic selenium species

Anal. Methods, 2026, 18,2429-2444
DOI: 10.1039/D5AY02045J, Paper

Huaqing Ling, Zhixi Zhao, Ziqi Zhang, Shan Chen, Zulihaya Maimaiti
PVA/AgNPs hydrogel SERS substrate via cyclic freeze-thaw: DFT and machine learning-assisted detection of organic selenium in soil and water.
The content of this RSS Feed (c) The Royal Society of Chemistry

M-Count: an application that uses machine learning object detection and color thresholding to count settled mussel larvae

Anal. Methods, 2026, 18,2395-2406
DOI: 10.1039/D5AY01759A, Paper

Open Access

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.

Lance W. Miller, Navaj Nune, Thomas B. LeFevre, Clare N. Hermanson, Joseph D. Daddona, Anita Valdez, Ashwin Kannan, Wilaiwan Chouyyok, George T. Bonheyo, Raymond S. Addleman, Curtis J. Larimer
Quantification of biofouling is a complex task that often involves counting both isolated and tightly packed groups of organisms on a test surface. M-Count quantifies mussel larvae fouling as accurately as humans but in a fraction of the time.
The content of this RSS Feed (c) The Royal Society of Chemistry