RSC - RSC Chem. Biol. latest articles

Probing Sulfotransferase Binding and Inhibition with Synthetic PAPS Analogs Reveals the Role of the 3′-Phosphate and Informs Molecular Tool Design

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00094K, Paper

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Maria Matveeva, Agnieszka Młynarska-Cieślak, Mikołaj Chromiński, Tomasz Spiewla, Jonathan Wolf Mueller, Jacek Jemielity, Joanna Kowalska
Sulfotransferases (STs) are a class of enzymes that catalyze the transfer of the SULFURYL GROUP (-SO₃−), most commonly from the universal cofactor 3′-phosphoadenosine 5′-phosphosulfate (PAPS), to nucleophilic acceptors bearing hydroxyl...
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Probing the C3 symmetry of Gramicidin S

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00134C, Communication

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Alex Hoose, Javier Garcia-Ruiz, Ciara C M Lally, Camilla Dondi, Maxim Ryadnov
Gramicidin S continues to inspire antibiotic designs. It exhibits a conserved two-fold (C2) symmetry postulated to underpin its biological activity. Here we probe the C3 symmetry of Gramicidin S and...
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Engineering a Light-Controllable Fluorescent Protein for Peroxynitrite Detection via Genetic Code Expansion

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00083E, Communication

Open Access

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Gloricelly Roman Arocho, Parthasarathi Das, Sachin Tennakoon, Jared Shaw, Wei Niu, Jiantao Guo
We report a noncanonical amino acid-containing photoconvertible fluorescent protein for selective peroxynitrite detection. Incorporation of p-borono-L-phenylalanine into circularly permuted mEos2 affords a peroxynitrite-responsive sensor that retains photoconversion capability, expanding chemical...
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Cellular Engagement of the SARS-CoV-2 Macrodomain by GS-441524 and Enhanced In Vitro Inhibition by its Diphosphorylated Metabolite

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00139D, Communication

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Banhi Biswas, Junlin Zhuo, Jürgen Bosch, Hien Vu, Lorencia Pak, Rameez Raja, Anuradha Roy, Alessandro Panattoni, Michal Maryska, Petr Slavík, Kryštof Šigut, Anthony R. Fehr, Rachy Abraham, Barbara Slusher, Takashi Tsukamoto, Anthony Leung
Coronavirus macrodomains (Mac1) counter host immunity by removing ADP-ribose. GS-441524, the parent nucleoside of remdesivir, inhibits SARS-CoV-2 Mac1, but its active metabolite and cellular engagement were undefined. GS-441524 selectively inhibits...
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Structure, characterisation and application of an unspecific peroxygenase from Daldinia childiae

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00141F, Paper

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Alexander McKenzie, Claudia Clark, Katy A. S. Cornish, Jiacheng Li, Jack Domenech, Benjamin Melling, Miles P. H. Ralston, Jared Cartwright, Nicholas P. Mulholland, William P. Unsworth, Gideon Grogan
The characterisation of the family I UPO from Daldinia childiae (DchUPO) is described, in addition to its application in small molecule oxygenation reactions.
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Reversible formation of S-D-3-phosphoglyceroyl glutathione contributes to cellular protection from acylation by cyclic 3-phosphoglyceric anhydride

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00085A, Paper

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Adilkhan Yeskendir, Adilet Bekmagambetov, Evelina Shkraba, Zariat Shatkenova, Zhanpeis Issayev, Aizhan Akhmadi, Dos D. Sarbassov, Darkhan Utepbergenov
Detoxification of cPGA in human cells: DJ-1 hydrolyzes the majority of cPGA molecules, while glutathione (GSH) captures the rest via thioester (pgGS) formation.
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Recent advances in near-infrared dye conjugates for near-infrared photoimmunotherapy (NIR-PIT): enhancing therapeutic efficacy and immune mechanisms

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00132G, Review Article

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Yoshikazu Fuse, Eita Sasaki, Orie Takayama, Sota Yamada, Kenjiro Hanaoka
Near-infrared photoimmunotherapy (NIR-PIT) is an innovative cancer treatment modality that was approved in Japan in 2020 for the treatment of unresectable locally advanced or locally recurrent head and neck cancer....
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Rational Design of an Acridine-derived Click Chemistry-based Artificial Metallo-Nuclease

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00097E, Paper

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Oliver Gould, Alex Gibney, Rebecca Lynn, Simon Poole, Brionna McGorman, Andrew Kellett
Artificial metallo-nucleases (AMNs) are metal complexes capable of cleaving nucleic acids and represent a promising therapeutic class. We recently established that copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) click chemistry offers a versatile...
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DNA Origami–Based Drug Delivery and Cell Manipulation: Toward Intelligent Nanomedicine

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00026F, Review Article

Open Access

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Yuki Suzuki
DNA origami has emerged as a versatile platform for constructing nanoscale architectures with precise shape programmability, molecular-level addressability, and dynamic structural reconfigurability. Since its introduction, DNA origami has evolved from...
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Stabilized thioamide peptide agonists of the neuropeptide Y type 2 receptor for targeted cancer imaging

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00042H, Paper

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Hoang Anh T. Phan, Yanan Chang, Taylor M. Barrett, Kristen E. Fiore, Daniel Y. Zhang, Ethan J. Grove, E. James Petersson
A fluorescent cyclic thioamide peptide has a serum half life over 8 hours and high selectivity for binding to Y₂R receptor expressing cells, enabling imaging of neuroblastomas and glioblastomas.
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A non-canonical function of RNF8 opposes TRAF6-mediated stabilization of HIF1α

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D5CB00209E, Paper

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Larissa Ringelstetter, Elisabeth Mersdorf-Weber, Kenji Schorpp, Kamyar Hadian
RNF8 antagonizes TRAF6-mediated stabilization of HIF1α via a non-canonical FHA-domain interaction, revealing a previously unrecognized mechanism that modulates hypoxia-induced signaling and downstream cellular responses.
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De novo grafted coiled-coil peptides as p53/hDM2 inhibitors

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00063K, Paper

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Freya Spain, Diana Gimenez, Amanda M. Acevedo-Jake, Bram Mylemans, Nikolas J. Brooks, Boguslawa Korona, Danny T. Huang, Thomas A. Edwards, Aneika C. Leney, Laura Itzhaki, Derek N. Woolfson, Andrew J. Wilson
Dysregulation of protein–protein interactions (PPIs) plays a key role in disease progression.
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Introducing azobenzenes as solid phase peptide synthesis linkers

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00028B, Communication

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Connor B. Śmieja, Lizun Xin, Tianhui Tang, Ryan Tan, Martin Lee, Alison N. Hulme
The ̲̲umarin-̲oaded ̲̲obenzene (COLAZ) linker for SPPS produces peptides with an integrated fluorescent label. Upon its biocompatible cleavage with dithionite or glutathione, the latent fluorophore is revealed enabling live-cell peptide imaging.
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ortho-Substituents govern aryl aldehyde reactivity: toward lysine-targeted, tunable inhibitors of glucose-6-phosphate dehydrogenase

RSC Chem. Biol., 2026, 7,1073-1087
DOI: 10.1039/D6CB00070C, Paper

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Bronwyn E. Rowland, Kelsey D. Roy, Adil Alkaş, Mirele Barsoum, Tangweth Kuot, Jesse C. Fuller, Adrien J. Naudet, Elisa Ospanow, David L. Jakeman
ortho-Substituents govern binding affinity and kinetics in aryl aldehyde imine formation. Varied physicochemical trends yield diverse mechanisms in glucose-6-phosphate dehydrogenase inhibition, supporting tunable, lysine-targeted inhibitor discovery.
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Stability modification of therapeutic aptamers: from biostability bottlenecks to nuclease-resistant construct design

RSC Chem. Biol., 2026, 7,964-982
DOI: 10.1039/D6CB00062B, Review Article

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Zhuoheng Pan, Yufei Pan, Huarui Zhang, Zefeng Chen, Yihao Zhang, Baoting Zhang, Aiping Lu, Ge Zhang, Sifan Yu
Nucleic acid aptamers are synthetic, single-stranded oligonucleotides that bind targets with high affinity and specificity, enabling precise and programmable functional modulation.
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Native MS and ligand observed NMR uncovers subtle SLiM binding variations that mediate HSP90-Hop PPI modulation

RSC Chem. Biol., 2026, 7,1099-1107
DOI: 10.1039/D6CB00112B, Paper

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Tara K. Davids, Daniel A. Kusza, Shannon K. Misplon, Josef C. Späth, Richwell Mhlanga, David J. Clarke, Beatriz G. de la Torre, Fernando Albericio, Adrienne L. Edkins, Marwaan Rylands, Clinton G. L. Veale
Orthogonal native mass spectrometry and ligand observed NMR elucidated subtle variations of SLiM binding TRP2A and provided a structural rationalization for HSP90-Hop PPI inhibition, and for future peptidomimetic optimisation.
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Correction: Dynamic conformational equilibria in the active states of KRAS and NRAS

RSC Chem. Biol., 2026, 7,1120-1121
DOI: 10.1039/D6CB90018F, Correction

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Enrico Rennella, Chrystèle Henry, Callum J. Dickson, Florian Georgescauld, Thomas E. Wales, Dirk Erdmann, Simona Cotesta, Michel Maira, Richard Sedrani, Saskia M. Brachmann, Nils Ostermann, John R. Engen, Lewis E. Kay, Kim S. Beyer, Rainer Wilcken, Wolfgang Jahnke
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A new 4-atom linker enables PROTAC development and imaging

RSC Chem. Biol., 2026, 7,1062-1072
DOI: 10.1039/D6CB00127K, Paper

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Spyros Letsios, Giovana Carrasco, Martin Lee, Mateen Wagiet, Marta Madureira, Marley Samways, Zaid Khan, Valerie G. Brunton, Olivera Grubisha, Alison N. Hulme
A rigid diyne linker gives a potent PROTAC that can be directly visualised in live cells by stimulated Raman scattering (SRS) microscopy.
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A fluorescence-activated droplet sorting assay for ultra-high-throughput screening of PET hydrolases based on a pH indicator

RSC Chem. Biol., 2026, 7,1056-1061
DOI: 10.1039/D6CB00122J, Paper

Open Access

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Maxine Yew, Haizhen Bi, Dingjie Wang, Rui Qi, Huiling Yuan, Huanhuan Zhai, Gen Li, Qinhong Wang, Leilei Zhu
Ultra-high-throughput screening of PET hydrolases enabled by a pH-based fluorescence-activated droplet sorting (FADS) assay.
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Prochelators modulate azole activity against Candida albicans in a metal-dependent manner

RSC Chem. Biol., 2026, 7,1006-1017
DOI: 10.1039/D6CB00073H, Paper

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Madeline Ann Merriman, Catherine A. Denning-Jannace, Andrea Barboza Hurtado, Francesca A. Vaccaro, Yu-Shien Sung, Elisa Tomat, Katherine J. Franz
Candida albicans is an opportunistic fungal pathogen of growing clinical concern, in part due to antifungal drug tolerance.
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Development of a spectrophotometric assay for high-throughput screening and mechanistic characterization of glucose-1-phosphate thymidylyltransferase inhibitors

RSC Chem. Biol., 2026, 7,1108-1119
DOI: 10.1039/D6CB00002A, Paper

Open Access

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Bronwyn E. Rowland, Jesse C. Fuller, Chigozie L. Okolie, David L. Jakeman
An assay was developed for high-throughput screening of thymidylyltransferase inhibitors. Precision and efficiency are improved compared to existing assays, enabling characterization of the inhibitor mechanism, as demonstrated using TDP-rhamnose.
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De novo acyl carrier proteins display structure-independent modification and sequence novelty

RSC Chem. Biol., 2026, 7,983-997
DOI: 10.1039/D5CB00148J, Paper

Open Access

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Michael A. Herrera, Grace K. King, Zoe Ozols, Gioele A. Tiburtini, Nicoletta Schiavo, Francesca Spyrakis, Louise K. Charkoudian, Dominic J. Campopiano
The creation of de novo acyl carrier proteins using a combined evolutionary and physicochemical approach.
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Mixed squaramide thioesters as phosphate group analogues for non-competitive antagonists of the phospholipid-sensing G protein-coupled receptor GPR55

RSC Chem. Biol., 2026, 7,1048-1055
DOI: 10.1039/D5CB00231A, Paper

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Junpei Abe, Xianyue Huang, Nozomi Ishii, Itaru Imayoshi, Yoshio Hirabayashi, Ichiro Matsuo, Hiroyuki Kamiguchi, Yukishige Ito, Adam T. Guy, Peter Greimel
High-affinity inhibitors of specific receptors are a valuable tool to elucidate cellular signaling, control biological systems and develop therapeutic drugs.
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Decoding protein-phospholipid interaction networks in cancer: the role of acyl-chain remodeling

RSC Chem. Biol., 2026, 7,933-944
DOI: 10.1039/D6CB00015K, Review Article

Open Access

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Berit Blume, Grace Potter, Carsten Schultz, Andrew Emili
Phospholipid acyl chain remodeling emerges as a chemical layer that rewires oncogenic signaling networks. Acyl chain-resolved lipidomics and multifunctional chemical probes enable mechanistic dissection of lipid–protein interaction networks.
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Backbone N-heteroatom substitution as a strategy to enhance peptide proteolytic stability

RSC Chem. Biol., 2026, 7,1042-1047
DOI: 10.1039/D6CB00061D, Paper

Open Access

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Avraz F. Anwar, Natalia Cano-Sampaio, Juan R. Del Valle
Peptide backbone N-amination confers significant resistance to proteolysis and enhances the conformation-dependent activity of an antimicrobial β-strand peptide.
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Igalan attenuates sepsis-induced inflammation through covalent targeting of the NLRP3 inflammasome pathway

RSC Chem. Biol., 2026, 7,1088-1098
DOI: 10.1039/D5CB00280J, Paper

Open Access

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Chengchen Hou, Yang Chen, Wenjie Bi, Lin Gao, Simiao Yu, Xiaowen Zhang, Zekun Chen, Tiantian Wei, Dilnoza E. Dusmatova, Rimma F. Mukhamatkhanova, Ildar D. Sham’yanov, Liwen Han, Zhiyuan Lu, Hua Wang, Nilufar Z. Mamadalieva, Kewu Zeng
Igalan covalently locks NLRP3 in an inactive state, blocking inflammasome activation and inflammatory cytokine release.
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Strategies to overcome hepatic clearance of endogenous proteins – molecular and formulation approaches

RSC Chem. Biol., 2026, 7,945-963
DOI: 10.1039/D6CB00041J, Review Article

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Kirstin Meiners, Tobias Stepic, Lorenz Meinel, Tessa Lühmann
This review examines advanced formulation and molecular engineering strategies to reduce rapid hepatic clearance of therapeutic proteins.
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Methylene insertion reveals the importance of phosphate–phosphate distance in DNase I cleavage

RSC Chem. Biol., 2026, 7,1034-1041
DOI: 10.1039/D6CB00024J, Paper

Open Access

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Ayano Nagaya, Kohji Seio, Yoshiaki Masaki
Phosphate recognition is important for DNase I activity.
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Enhancing mRNA stability and translational potential through tailored modifications at the 3′ end

RSC Chem. Biol., 2026, 7,1024-1033
DOI: 10.1039/D6CB00033A, Paper

Open Access

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Olga Perzanowska, Joanna Kowalska, Jacek Jemielity
Small and targeted modification was introduced at the 3′ end of mRNA to increase its stability and translational efficiency. This strategy resulted in up to a 2.5-fold increase in protein output in cells.
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RNA-binding landscape of amiloride: large-scale profiling and structural basis of U–U mismatch recognition

RSC Chem. Biol., 2026, 7,998-1005
DOI: 10.1039/D6CB00055J, Paper

Open Access

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Kosuke Tsuzuki, Kazumitsu Onizuka, Momo Okada, Ryosuke Nagasawa, Emi Miyashita, Kaoru R. Komatsu, Hirohide Saito, Jiro Kondo, Fumi Nagatsugi
Comprehensive FOREST profiling identified a U–U mismatch RNA motif to which amiloride strongly binds. X-ray crystallography revealed the precise binding mode, driven by three complementary hydrogen bonds with uracil and stacking interactions.
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A cysteine reactive chloroalkane probe enables HaloTag ligation for downstream chemical proteomics analysis

RSC Chem. Biol., 2026, 7,1018-1023
DOI: 10.1039/D6CB00004E, Paper

Open Access

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Rubaba R. Abanti, Dongqing Wu, Pavel Kielkowski
Abanti et al. describe the application of the HaloTag protein in a chemical proteomics workflow. HaloTag facilitates fast and highly specific conjugation with probe labelled proteins, allowing downstream shift-assay, pull-down and proteomics.
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Thio-modification effects on mRNA translation using a PureCap-based capping method

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00047A, Communication

Open Access

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Zheyu Meng, Yiwei Liu, Yuko Nakashima, Masahito Inagaki, Seigo Kimura, Yukari Kondo, Fumitaka Hashiya, Naoko Abe, Noriko Saito-Tarashima, Noriaki Minakawa, Yasuaki Kimura, Hiroshi Abe
Sulfur-modified PureCap analogs enable HPLC purification of the corresponding thio-modified mRNAs, allowing accurate evaluation of 4′-thio and phosphorothioate modification effects in cells and in vivo.
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Methylation of DNA duplexes regulates cGAS-mediated innate immune activation via condensate formation

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00059B, Communication

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Kunihiko Morihiro, Manami Baba, Moeko Yamada, Akimitsu Okamoto
Systematic comparison of DNA chemical modifications identifies 2′-OMe-modification as a potent enhancer of cGAS-mediated condensate formation and innate immune signaling.
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The inhibitors of 17β-HSD10: are they any good?

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00093B, Review Article

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Ernst Kwa, Charlene E. Ogilvie, Natalie C. Kormos, Alison J. E. Green, Terry K. Smith, Frank J. Gunn-Moore
The advent of the first disease-modifying therapies for Alzheimer's disease (AD) has renewed optimism for effective prevention and treatment strategies. One particular strategy is to target the mitochondrial protein 17β-HSD10 as its activity has been linked to several aspects in AD pathogenesis.
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Precursor gene engineering expands the loop region of the lasso peptide microcin J25

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00126B, Communication

Open Access

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Hui-Ni Tan, Julian D. Hegemann, Manuel Maestre-Reyna, John Chu
The lasso peptide microcin J25 has a unique mechanically interlocked structure. We show that multiple amino acids of diverse types can be inserted into its loop region via precursor gene engineering.
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Peptides as programmable molecular scaffolds: from chemical synthesis and engineering to translational medicine

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00117C, Review Article

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Shaoren Yuan, Baljit Kaur, Natalie S. Fuchs, Sungwoo Cho, Ashraf N. Abdo, Moustafa T. Gabr
Peptides have evolved from naturally occurring ligands and classical hormones into a versatile and engineerable class of functional molecules.
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The γ-Secretase Complex: From Discovery to Therapeutic Target

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00022C, Review Article

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Shweta R. Malvankar, Michael Scott Wolfe
γ-Secretase is an intricate intramembrane aspartyl protease that cleaves within the transmembrane domain of ~150 substrates and considered the 'proteasome of membrane'. This enzyme consists of four different subunits, with...
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Exploring the GM-CSF histidine triad as a modulator of structure, molecular motion, and ligand binding

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00019C, Paper

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Jennifer Y. Cui, Iz Varghese, Anna S. Bock, Mariana Floody, Fuming Zhang, Brenda M. Rubenstein, George P. Lisi
Can mutations in the GM-CSF histidine triad mimic the structural, dynamic and binding properties of its acidified form at neutral pH?
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Pharmacology of estrogen-related receptors (ERRs) agonists

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00077K, Review Article

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Mohamed O. Radwan, Soumitra Guin, Thomas P. Burris
Recent advances in the development of synthetic agonists for the orphan receptors, estrogen-related receptors (ERRs), has revealed their exercise mimetic activity and beneficial effects in skeletal muscle, heart, and brain.
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Minimal N-methylated and stapled peptide inhibitors of the autophagy protein GABARAP

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00111D, Paper

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Imani McDonald, Joana A. Wilms, Nicholas Cardi, Alexander Engstrom, Jiayuan Miao, Dieter Willbold, Yu-Shan Lin, R. Scott Lokey, Oliver H. Weiergräber, Joshua A. Kritzer
Ligands for the autophagy protein GABARAP are highly sought-after as autophagy modulators and components of targeted degraders. This work developed passively penetrant GABARAP ligands using peptide stapling and N-methylation.
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Understanding insulin-like peptide 5 (INSL5) and relaxin family peptide receptor 4 (RXFP4): structure, signalling, and function

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00102E, Review Article

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Hongkang Wu, Huahao Wang, Isabelle Riches, Ivan Maslov, Predrag Kalaba, Myat Noe Han, Ruslan Pustovit, John B. Furness, Ross A. D. Bathgate, Mohammed Akhter Hossain
This review summarises current knowledge of the insulin-like peptide 5 (INSL5) and relaxin family peptide receptor 4 (RXFP4) system, as well as recent advances in the discovery of peptidomimetics and small-molecule ligands targeting this pathway.
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A two-step bioconjugation of S. aureus lipoteichoic acid (LTA) affords fluorescent probes that illuminate the interaction between Gram-positive glycolipids and mammalian cell membranes

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00113K, Paper

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Jake Henry, Joe Nabarro, Harriet Chidwick, Georgia Conrich-Wilks, Emily G. Morton-West, Alice Crook, Dave Boucher, Nathalie Signoret, Martin A. Fascione
Oxime-based bioconjugation enables fluorescent labelling of native bacterial glycolipid lipoteichoic acid (LTA), facilitating study of its interaction with mammalian membranes.
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The position of hydrophobic residues impacts cellular uptake and intracellular localization of cell penetrating peptides

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00107F, Paper

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Adeline Schmitt, Helma Wennemers
Hydrophobic residues at the C-terminus of cationic cell-penetrating peptides (CPPs) enhance internalization and endosomal release. This motif can also facilitate delivery to mitochondria and the endoplasmic reticulum.
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Scarless one-tube genome assembly via computationally optimized uracil-DNA glycosylase reactions

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D5CB00317B, Paper

Open Access

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Rachel M. Carson, Patrick M. Needham, Pedro J. Fernandez Mendoza, Sam R. Nugen
Synthetic biology enables the creation of systems such as bacteriophage (phage)-based biosensors, leveraging the innate specificity and efficiency of phages to rapidly identify pathogens.
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Real-time monitoring of glycocarrier formation unravels cryptic details in glycosyl transfer

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00069J, Paper

Open Access

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Ryan D. Packer, Angelo Gallo, Alexander D. Cameron, Somnath Mondal, Józef R. Lewandowski, Manuela Tosin
A real-time fluorescence-based assay has been developed to unveil molecular details of glycocarrier formation in health and disease.
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Synthesis and characterization of a novel phosphatidylinositol 5-phosphate (PI(5)P) photoaffinity probe

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00148C, Paper

Open Access

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Glen Brodie, Ahmed Sayed, Sarah Kreuz, Wolfgang Fischle, Stuart J. Conway
A novel PI(5)P photoaffinity probe was synthesized. The probe was characterized in copper-catalyzed alkyne-azide click reactions with the known PI(5)P nuclear binding proteins hUHRF1 and hTAF3 using dyes and affinity tags.
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Cooperative membrane association as a mechanistic origin of synergistic antimicrobial peptide activity

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00084C, Review Article

Open Access

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Angela Medvedeva, Anatoly B. Kolomeisky
AMP–AMP combinations are tested through checkerboard assays, and their antibacterial properties are measured. Synergy occurs at AMP concentrations that are shown by striped green circles.
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Tipping the balance: synthesis and evaluation of centrinone-based degraders of polo-like kinase 4

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D5CB00315F, Paper

Open Access

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Andrej Kovacevic, Aleksandar Salim, Crisálida Borges, Patrick Meraldi, Sascha Hoogendoorn
Bifunctional degraders based on the PLK4 inhibitor centrinone exhibit diverging cellular profiles of stabilization through inhibition and degradation through a PROTAC-mechanism – depending on the cellular localization of PLK4.
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Protein Digestion Using a Cysteine-Specific Backbone Cleavage Reagent

RSC Chem. Biol., 2026, Accepted Manuscript
DOI: 10.1039/D6CB00152A, Communication

Open Access

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Yu-De Chuang, Tsung-Jung Yang, Yu-Chi Chih, Po-Cheng Kuo, Cheng-Chih Hsu, Shao-Lun Chiou, John Chu
Mass spectrometry (MS)-based protein analysis is an indispensable tool in modern biomedical research. A key step in sample preparation is proteolytic digestion using enzymes with well-defined amino acid specificity, such...
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Application of the YebF secretion pathway in Escherichia coli for rapid, on-plate screening of PETase libraries for improved activity

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00090H, Paper

Open Access

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Jaeick Lee, Maame Yaa Yamoa, Celina L. Bradley, Graeme Howe, David L. Zechel
YebF-mediated secretion enables the rapid, on-plate screening of PETase libraries in E. coli, identifying LCC variants with enhanced PET hydrolytic activity through zone-clearing assays.
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Let the peptides shine: SOX (Sulfonamido-OXine)-labelled peptides for direct kinase and phosphatase monitoring

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00048G, Review Article

Open Access

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Lydia E. Papagora, Stephen A. Cochrane
SOX-labelled peptides enable direct, continuous fluorescence readouts of kinase and phosphatase activity. These CHEF-based probes provide real-time enzymatic assays in purified systems and cell lysates.
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The working lives of neuronal 5-HT2A receptors from the outside in

RSC Chem. Biol., 2026, Advance Article
DOI: 10.1039/D6CB00014B, Review Article

Open Access

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Aneesah Jabar, Mikayla Pinto, Terence E. Hébert
Signalling downstream of 5-HT_2A receptors may be driven by biased or location-biased ligands that will modulate the nature and kinetics of signals that may help discriminate between hallucinogenic and non-hallucinogenic receptor responses.
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